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袋栽香菇接种后,一般都要在穴口上贴胶布,不但增加接种时的工作量,而且胶布经蒸汽灭菌后容易脱胶或老化,杂菌即趁虚而入,从而降低了成品率。为解决这个问题,笔者采取用香菇木屑菌种直接封实穴口法,几经小试取得了成功,并于87年秋在我县5个香菇袋栽生产点推广应用,共接种53600多个菌筒,成品率平均达80%以上,最高的达93.1%。现将具体方法介绍如下:首先要制一根长15厘米、直径2厘米的锥形木棒。将经灭菌的料袋连同木棒一起放入接种箱(室)内进行消毒。接种时木棒和料袋的接种部位均要用75%酒精棉球揩擦消毒,再用木棒的尖端打一个深2厘米的穴,然而接入香菇木屑菌种揿严,并与菌种料袋表面相平。由于菌种与栽培料紧密结合,再加上不贴胶布透气性好,所以菌丝定植快,抵抗杂菌能力强。
Bag mushrooms inoculated, generally have to stick tape on the hole, not only increase the workload of vaccination, and tape easily after steam sterilization degumming or aging, bacteria that take advantage of, thus reducing the yield. In order to solve this problem, the author adopted the method of direct sealing and stomatism with Shiitake mushrooms, and succeeded several times in small trials. In the autumn of 1987, it popularized and applied 5 mushroom bag planting points in our county, inoculating more than 53600 bacteria cartridges, The average finished product rate of more than 80%, the highest of 93.1%. The specific methods are introduced as follows: First of all, to make a length of 15 cm, 2 cm in diameter tapered sticks. Put the sterilized material bag into the inoculation box (room) along with the sticks for disinfection. Inoculation of sticks and bags of vaccination sites should use 75% alcohol tampon rub disinfection, and then use the tip of a wooden stick to play a 2 cm deep hole, however, access to Shiitake Shiitake mushroom species and strict with the strains Material bag surface phase flat. As the strains and planting materials in close contact, coupled with good adhesive tape is not breathable, so fast colonization mycelium, resistant to bacteria and strong ability.