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目的探讨T-cadherin基因在前列腺癌组织中的表达及其对前列腺癌细胞DU145增殖的影响。方法采用RT-PCR法检测40份前列腺癌及相应癌旁组织中T-cadherin基因mRNA的表达。分别用10 ml滴度为1.6×10~(12)pdf/ml的GFP-T-cadherin腺病毒(Ad-GFP-T-cadherin)和GFP腺病毒(Ad-GFP)感染前列腺癌DU145细胞后,MTT法检测T-cadherin对前列腺癌DU145细胞增殖的影响,Western blot法检测T-cadherin对P21和cyclin D1蛋白表达水平的影响,同时设空白对照组(未感染病毒)。结果 T-cadherin在38/40(95%)的前列腺癌中表达下调(P<0.01),其表达与前列腺癌的分期、格里森评分及分化有关。Ad-GFP-T-cadherin组DU145细胞中P21蛋白表达水平明显高于Ad-GFP组及空白对照组(P<0.01),而cyclin D1蛋白表达水平及增殖活性明显低于Ad-GFP组及空白对照组(P<0.01);空白对照组DU145细胞的增殖活性及细胞中P21、cyclin D1蛋白表达水平与Ad-GFP组比较,差异无统计学意义(P>0.05)。结论 T-cadherin的表达与前列腺癌的发生密切相关,且可通过上调P21和下调cyclin D1蛋白的表达抑制前列腺癌DU145细胞的增殖。
Objective To investigate the expression of T-cadherin gene in prostate cancer tissue and its effect on the proliferation of prostate cancer cell line DU145. Methods The expression of T-cadherin mRNA was detected by RT-PCR in 40 specimens of prostate cancer and corresponding paracancerous tissues. Prostate cancer DU145 cells were infected with 10 ml GFP-T-cadherin and GFP adenovirus (Ad-GFP) with a titer of 1.6 × 10 ~ (12) pg / The effect of T-cadherin on the proliferation of prostate cancer DU145 cells was detected by MTT assay. The effect of T-cadherin on the expression of P21 and cyclin D1 protein was detected by Western blot, and the blank control group (non-infected) was also established. Results T-cadherin was down-regulated in 38/40 (95%) of the prostatic cancers (P <0.01). The expression of T-cadherin correlated with the staging, Gleason score and differentiation of prostate cancer. The expression of P21 protein in DU145 cells of Ad-GFP-T-cadherin group was significantly higher than that in Ad-GFP group and blank control group (P <0.01), while the expression of cyclin D1 protein and proliferation activity was significantly lower than that of Ad-GFP group (P <0.01). The proliferative activity of DU145 cells and the expression of P21 and cyclin D1 in DU145 cells in blank control group were not significantly different from those in Ad-GFP group (P> 0.05). Conclusions The expression of T-cadherin is closely related to the occurrence of prostate cancer, and can inhibit the proliferation of prostate cancer DU145 cells by up-regulating P21 and down-regulating the expression of cyclin D1.