Realgar-Induced Apoptosis of Cervical Cancer Cell Line Siha via Cytochrome C Release and Caspase-3 a

• 作 者: 程艳香 刘嵘 王琴 李秉枢 许学先 胡敏 陈璐 付琼 濮德敏 洪莉
• 机 构: Department of Gynecology,Renmin Hospital of Wuhan University,Department of Obstetrics and Gynecology
• 出 处: Chinese Journal of Integrative Medicine
• 发表日期: 2012年05期
• 关键词: caspase Caspase Apoptosis cytochrome cytometry sequentially inhibited manner acc

Objective:To explore the molecular mechanism of realgar-induced apoptosis of cervical cancer cells.Methods:The cervical cancer cell line Siha was used to determine the cell viability and apoptosis after treatment with realgar using MTT assay and flow cytometry.The activities of caspase-3,-8,and-9 were detected by fluorescence resonance energy transfer technology and colorimetric assay,while the levels of Bcl-2,cytochrome c,and Bax were detected by Western blot method.Results:Induction of apoptosis by realgar was detected in Siha cell line in a dose-dependent manner.The apoptosis was accompanied by a significant increase in cytochrome c release and activation of caspase-3 and caspase-9 but not caspase-8.Further,the realgar-induced apoptosis was inhibited by a broad-spectrum caspase inhibitor,a caspase-3 inhibitor,and a caspase-9 inhibitor but not by a caspase-8 inhibitor.Bcl-2 and Bax protein expressions were not changed by realgar.Conclusion:The induction of apoptosis by realgar is mediated through a cytochrome c-dependent pathway,which sequentially activates caspase-9 and caspase-3.

Objective: To explore the molecular mechanism of realgar-induced apoptosis of cervical cancer cells. Methods: The cervical cancer cell line Siha was used to determine the cell viability and apoptosis after treatment with realgar using MTT assay and flow cytometry. Activities of caspase- 3, -8, and-9 were detected by fluorescence resonance energy transfer technology and colorimetric assay, while the levels of Bcl-2, cytochrome c, and Bax were detected by Western blot method. Results: Induction of apoptosis by realgar was detected in Siha cell line in a dose-dependent manner. The apoptosis was accompanied by a significant increase in cytochrome c release and activation of caspase-3 and caspase-9 but not caspase-8.Further, the realgar-induced apoptosis was inhibited by a broad -spectrum caspase inhibitor, a caspase-3 inhibitor, and a caspase-9 inhibitor but not by a caspase-8 inhibitor. bcl-2 and Bax protein expressions were not changed by realgar. Confluence: The induction of apoptosis by realgar is med iated through a cytochrome c-dependent pathway, which were activates caspase-9 and caspase-3.