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OBJECTIVE To investigate slow-release features of biodegradable anticancer 5-fluorouracil-loaded immunonanoparticles (5-FU INPs), and to assess their tumor cell killing activity in vitro. METHODS The method of vibrating dialysis at a constant temperature, and first-order derivative ultraviolet spectrophotometry were used to deter- mine the drug-releasing character of 5-FU INPs. The methyl thiazolyl tetrazo- lium (MTT) colorimetric method was employed to assay the killing activity of 5-FU INPs on 5 tumor cell lines at different phases. RESULTS The 5-FU INPs had a favorable slow-release function, with a ot1n/2 r5e lteuamseo rt icmeell olifn 1e0s. 4re dsauyltsin. gT hine 5a- FpUos IiNtivPes choardre ala rtaivtihtye rb setrtwonege nle tthhael keiflfleincgt activity and the action time and amount of the drug released. CONCLUSION The drug disposition is uniform from the 5-FU INPs, and there is no impact on efficacy of the 5-FU during preparation and degra- dation of the 5-FU INPs. The 5-FU INPs have a favorable function for drug release, and can maintain an effective killing activity over a long period of time.
OBJECTIVE To investigate slow-release features of biodegradable anticancer 5-fluorouracil-loaded immunonanoparticles (5-FU INPs), and to assess their tumor cell killing activity in vitro. METHODS The method of vibrating dialysis at a constant temperature, and first- ultraviolet spectrophotometry were used to deter- mine the drug-releasing character of 5-FU INPs. The methyl thiazolyl tetrazo- lium (MTT) colorimetric method was employed to assay the killing activity of 5-FU INPs on 5 tumor cell lines at different . RESULTS The 5-FU INPs had a favorable slow-release function, with a ot1n / 2 r5e lteuamseo rt icmeell olifn 1e0s. 4re dsauyltsin. GT hine 5a- FpUos IiNtivPes choardre ala rtaivtihtye rb setrtwonege nle tthhael keiflfleincgt activity and the action time and amount of the drug released. CONCLUSION The drug disposition is uniform from the 5-FU INPs, and there is no impact on efficacy of the 5-FU during preparation and degradation of the 5-FU INPs. The 5-FU I NPs have a favorable function for drug release, and can maintain an effective killing activity over a long period of time.