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试验研究分析了小麦在不同氮素水平下氮素和叶绿素计值(SPAD,SoilandPlantAnalyzer Development)的叶位分布特点,以及小麦叶片氮素含量和SPAD值的相关性,探讨应用SPAD-502进行小麦氮素营养诊断的理论依据。通过沙培试验,利用叶绿素仪SPAD-502测定了不同氮素水平小麦(新春11号)在不同生育时期(分蘖期、拔节期)叶片的SPAD值、叶绿素含量及植株全氮含量,在此基础上分析了小麦叶片的SPAD值与植株叶绿素含量、全氮含量的关系,目的在于为SPAD快速诊断施肥法在小麦等作物上的应用提供理论依据。结果表明:叶绿素与小麦不同叶位叶片SPAD值含量呈显著正相关;全氮含量与小麦叶片SPAD值呈正相关,因此,可以用SPAD值估算全氮含量,从而为小麦氮素营养状况提供诊断,但是全氮含量与不同叶位SPAD值的关系表现不一致。
This study analyzed the leaf position distribution characteristics of wheat and nitrogen at different nitrogen levels (SPAD, Soiland Plant Anaerzer Development), and the correlation between nitrogen content and SPAD value of wheat leaves. The application of SPAD-502 in wheat nitrogen Vegetarian diagnosis of the theoretical basis. The SPAD value, chlorophyll content and plant total nitrogen content in different growth stages (tillering stage and jointing stage) of wheat with different levels of nitrogen (Xinchun 11) were determined by using sand chlorophyll assay SPAD-502. The relationship between the SPAD value of wheat leaves and the chlorophyll content and total nitrogen content of the plants was analyzed. The purpose was to provide a theoretical basis for the application of the SPAD rapid diagnostic fertilization method on crops such as wheat. The results showed that there was a significant positive correlation between chlorophyll content and SPAD value in wheat leaves. Total nitrogen content was positively correlated with SPAD value in wheat leaves. Therefore, SPAD value could be used to estimate total nitrogen content, However, the relationship between total nitrogen content and SPAD values at different leaf positions was inconsistent.