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获得鱼类雄核发育后代有2种方法。一种是用正常精子使经过遗传失活的卵受精,得到雄核发育单倍体胚胎,然后通过抑制第1次卵裂的发生诱导核内有丝分裂,使单倍体胚胎的染色体加倍发育为纯合二倍体个体;另一种方法是用四倍体个体的精子使遗传失活的卵子受精,直接得到正常发育的二倍体雄核发育后代。介绍了雄核发育技术的基本原理和基本技术手段,探讨了雄核发育技术在鱼类遗传育种研究中的应用现状。
There are 2 ways to get descendants of fish male pronucleus. One is the use of normal sperm to genetically inactivate the eggs by fertilization, get the male embryo development of haploid embryos, and then by inhibiting the occurrence of the first cleavage induced nuclear mitosis, the haploid embryo chromosome doubling developed pure And diploid individuals; the other method is to fertile the genetically inactivated egg with the tetraploid individual’s sperm to directly obtain the normal diploid progeny of the male parent. Introduced the basic principles and basic techniques of the technology of the male development of nucleus, and discussed the application of the technology of the male nucleus development in the research of fish genetics and breeding.