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目的:通过对糖尿病大鼠模型早期视觉功能的变化和糖尿病视网膜的蛋白质氧化损伤进行观察,为研究糖尿病视网膜病变发病机制提供实验依据。方法:实验于2002-10/2004-05在南京医科大学生理学教研室完成。用图形视网膜电图(patternelectroretinogram,PERG)、闪光视网膜电图(flashelectroretinogram,FERG)分别记录正常和链脲菌素(streptozotocin,STZ)诱导的糖尿病大鼠8周以内PERG-q波及FERG-b波潜时,观察糖尿病大鼠早期视网膜功能的改变;以及用免疫组化法和图像处理检测糖尿病大鼠视网膜组织蛋白质损伤产物3-硝基酪氨酸(3-Nitrotyrosine,3-NT)的表达及其改变。结果:糖尿病大鼠从模型建立后第3天开始PERG-q波潜时与正常大鼠相比即有显著延长(P<0.001),而从第1周开始FERG-b波的潜时与正常大鼠相比也显著延长(P<0.001),并且PERG-q波潜时的改变早于FERG-b波潜时的改变;正常大鼠视网膜内无3-NT免疫反应阳性细胞,在糖尿病大鼠视网膜,3-NT阳性反应主要发生在神经节细胞层(ganglioncelllayer,GCL)、并且内核层和外核层均有存在,图像处理显示,糖尿病2,4和8周大鼠视网膜3-NT阳性细胞数差异无显著性意义(P>0.05)。结论:糖尿病大鼠早期在未发现眼底可见的视网膜病变之前就有可用视觉电生理方法检测到的视功能异常,并且糖?
OBJECTIVE: To observe the changes of early visual function in diabetic rat model and the protein oxidative damage of diabetic retina, and provide experimental evidence for studying the pathogenesis of diabetic retinopathy. Methods: The experiment was performed at Department of Physiology, Nanjing Medical University from October 2002 to May 2004. The levels of PERG-q and FERG-b in normal and streptozotocin (STZ) -induced diabetic rats were recorded with the pattern electroretinogram (PERG) and flashelectroretinogram (FERG) , The changes of early retinal function in diabetic rats were observed, and the expression of 3-nitrotyrosine (3-NT) protein in retinal tissue of diabetic rats was detected by immunohistochemistry and image processing change. Results: From the third day after model establishment, the latency of PERG-q wave in diabetic rats was significantly longer than that in normal rats (P <0.001), while the latency and normality of FERG-b wave from the first week (P <0.001), and the change of PERG-q wave latency was earlier than that of FERG-b wave latency. No normal 3-NT immunoreactive cells were found in the retina of normal rats. The retinal 3-NT positive reaction mainly occurred in the ganglion cell layer (GCL), and both the inner and outer nuclear layers were present. Image processing showed that retinal 3-NT was positive in diabetic rats at 2, 4 and 8 weeks No significant difference in cell number (P> 0.05). CONCLUSION: There are visual abnormalities detected by visual electrophysiology before diabetic retinopathy is found in diabetic rats.