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非Ｏ１群霍乱弧菌（不包括Ｏ１３９菌）是腹泻病原菌，由于它们分布较广，因此对人们的健康具有较大的威协；应用核酸技术对９８株非Ｏ１菌的主要毒力基因进行检测，并从ＤＮＡ和基因水平对５株非Ｏ１菌的分子特征进行研究。结果：所选菌株的染色体酶切图谱与Ｏ１群霍乱弧菌相比有很大差异，尤其与Ｏ１群流行株的差异更大，非Ｏ１菌相互之间的差异也很大；对９８株非Ｏ１菌进行ｃｔｘ、ｚｏｔ、ａｃｅ、ＲＳ１基因检测，结果有２株为上述基因阳性，仅为２％左右。结果显示：非Ｏ１菌群体遗传结构复杂，变异程度高；其与Ｏ１菌，尤其是流行株（包括Ｏ１３９菌）的遗传关系较远；结果也说明大多数非Ｏ１菌是不致病的
Non-O1 group Vibrio cholerae (excluding O139 bacteria) are pathogenic bacteria of diarrhea, because they are widely distributed, and therefore have a strong threat to people’s health; using nucleic acid technology to detect the major virulence genes of 98 non-O1 bacteria The molecular characteristics of five non-O1 bacteria were studied at the DNA and genetic level. Results: The results showed that there was a great difference between the isolates of Cholera O1 and Vibrio cholerae O1 strains, especially the difference between the O1 strains and the non-O1 strains. O1 bacteria ctx, zot, ace, RS1 gene test, the results of 2 strains of the above genes were positive, only about 2%. The results showed that the genetic structure of non-O1 strains was complex and the degree of variation was high; the genetic relationship with O1, especially the epidemic strains (including O139) was far. The results also showed that most non-O1 strains were not pathogenic