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目的探讨血清肿瘤坏死因子α(TNFα)水平及TNFα启动子基因多态性与巨细胞病毒(CMV)感染所致婴儿肝炎综合征(NHS)的关系。方法采用酶联免疫吸附试验(ELISA)检测血清TNFα水平,同时应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TNFα启动子基因多态性。结果在NHS患儿中,TNFα基因启动子区-308 G-A单碱基突变频率明显高于健康人群(P<0.01),在NHS患儿母亲中的突变频率也明显高于健康人群(P<0.01),在NHS患儿与NHS患儿母亲中的突变频率差异无显著性。NHS患儿血清TNFα水平[(1 164.00±576.00)pg/m l]显著高于正常同龄婴幼儿[(5.22±2.24)pg/m l,P<0.001];NHS患儿母亲血清TNFα水平[(822.60±506.00)pg/m l]显著高于正常健康成年人[(14.90±8.20)pg/m l,P<0.001],各组内TNFα基因型间TNFα水平比较差异均无显著性(P>0.05)。结论TNFα的基因启动子区-308单碱基多态性可能与NHS的易感性、发病及致病相关。
Objective To investigate the relationship between serum tumor necrosis factor-α (TNFα) and TNFα promoter polymorphism and infantile hepatitis syndrome (NHS) induced by cytomegalovirus (CMV) infection. Methods Serum TNFα levels were measured by enzyme-linked immunosorbent assay (ELISA). The polymorphism of TNFα promoter was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results In NHS children, the single nucleotide mutation of -308 GA in TNFα gene promoter region was significantly higher than that in healthy people (P <0.01), and the frequency of mutation in mothers with NHS was also significantly higher than that in healthy people (P <0.01) ), There was no significant difference in the frequency of mutation among mothers of NHS children and NHS children. The level of serum TNFα in children with NHS [(1 164.00 ± 576.00) pg / ml] was significantly higher than that in normal infants (5.22 ± 2.24 pg / ml, P <0.001) 506.00) pg / ml] was significantly higher than that in healthy adults [(14.90 ± 8.20) pg / ml, P <0.001]. There was no significant difference in the TNFα levels between groups (P> 0.05). Conclusion TNF-α gene promoter region -308 single base polymorphism may be associated with the susceptibility to NHS, pathogenesis and pathogenesis.