IFN-γ基因转染膀胱癌瘤苗诱导的特异性抗肿瘤免疫反应

• 作 者: 辛军 王晓雄 孟书礼 王鑫 刘爽 刘玉晶
• 机 构: 解放军总医院泌尿外科
• 出 处: 中华泌尿外科杂志
• 发表日期: 1998年07期
• 关键词: 膀胱癌细胞 IFN 瘤苗 膀胱肿瘤 抗肿瘤免疫 基因转染 抗原表达 皮下接种 脾细胞 自分泌

探讨转ＩＦＮγ基因膀胱癌瘤苗的可行性。采用脂质体介导法将携带鼠ＩＦＮγ基因的逆转录病毒载体转染入鼠膀胱癌细胞系ＢＴＴ７３９中，免疫荧光法检测转基因细胞ＭＨＣＩ类抗原表达；动物实验观察其致瘤性和抗肿瘤免疫功能。结果显示：建立能自分泌ＩＦＮγ的膀胱癌瘤苗ＢＴＴ７３９／ＩＦＮγ，瘤苗细胞ＭＨＣＩ类抗原表达量明显增加，致癌性显著降低，并可诱导机体产生特异性抗肿瘤免疫，ＣＤ＋８Ｔ淋巴细胞在抗肿瘤免疫过程中起重要作用。结果证明：转ＩＦＮγ基因膀胱癌瘤苗可诱导机体产生特异性抗肿瘤免疫反应

To explore the feasibility of transfer of IFN γ gene bladder cancer vaccine. The retroviral vector carrying murine IFNγ gene was transfected into the mouse bladder cancer cell line BTT739 by liposomemediated method. The expression of MHC class I antigen in transgenic cells was detected by immunofluorescence. The tumorigenicity and anti-tumor activity Tumor immune function. The results showed that the expression of MHC class I antigen in tumor cells significantly increased after establishment of the bladder cancer vaccine BTT739 / IFNγ secreting IFNγ, the oncogenicity was significantly reduced, and the specific antitumor immunity was induced in the body. CD + 8T lymphocytes Cells play an important role in anti-tumor immunity. The results show that: transfer of IFN γ gene bladder cancer vaccine can induce the body to produce specific anti-tumor immune response