Enzyme-free amplified detection of micro RNA using target-catalyzed hairpin assembly and magnesium i

• 作 者: Jinfeng Chen Bo Liu Xiaorong Song Ping Tong Huanghao Yang Lan Zhang
• 机 构: Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Key Laboratory of
• 出 处: Science China(Chemistry)
• 发表日期: 2015年12期
• 关键词: hairpin magnesium assembly catalyzed RNA selectivity perfectly preferred facilit

As micro RNAs(mi RNAs) are aberrantly expressed in a variety of cancers, detecting them precisely is of great importance. Here we constructed a sensitive and selective enzyme-free sensing platform for mi RNA detection based on target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme(Mg2+-dependent DNAzyme). This sensing method introduces two amplification circuits simultaneously and shows a low detection limit of 1 pmol/L. This enzyme-free method is especially preferred because of its facility and economy. Furthermore, this amplified sensor shows high selectivity for discriminating perfectly complementary target and other mismatched RNAs. Therefore, the established strategy could be used as a simple, sensitive and selective method for target mi RNA detection.

As we are a sensitive and selective enzyme-free sensing platform for miRNA detection based on target-catalyzed hairpin assembly and magnesium ion This sensing method introduces two amplification cycles simultaneously and shows a low detection limit of 1 pmol / L. This enzyme-free method is especially preferred because of its facility and economy. Furthermore, this amplified sensor shows high selectivity for discriminating perfectly complementary targets and other mismatched RNAs. Thus, the established strategy could be used as a simple, sensitive and selective method for target mi RNA detection.