论文部分内容阅读
1 材料和方法1.1 采用新鲜尸头,非中枢神经系统疾病死亡,一般在死亡后7小时内灌注.1.2 铸型剂的预聚合 用9份甲基丙烯酸甲酯(Methylmethacrylate)混以1份丙烯酸甲酯(Methyl-crylate增韧剂),并加入上述二液总量的6%过氧化苯甲酰(Benjoylperoxide引发剂).将上述混合液放入锥形瓶内,水浴加热至78～80℃.当浴液粘稠度呈25～30%水甘油样浓度,颜色微黄而透明时即完成预聚合,迅速冷却.单体内若含有对苯二酚等阻聚剂,预聚合前用1～2%NaOH洗涤,去阻聚剂,再以蒸馏水洗至呈中性,用分子筛(活性碳)去水分.1.3 树脂灌注 将新鲜尸头取下,经双侧颈内动脉及椎动脉插管,用生理盐水冲洗至静脉流出清水,先以0.5%戊二醛30～40ml灌流固定血管内皮,然后再以单体引路,将预聚合体混以少量1%二甲基苯胺(Dimethylanilin促进剂),用注射器注入,压力适中,直至由颈内静脉及椎动脉流出预聚合体,再置尸头于50～60℃水中浸泡数小时,可保持原形,也有助于树酯硬化.1.4 取材 待铸型硬经后开颅取脑(一般约2～3天).先按欲观察之部位取材,大小视要求而定.1.5 组织的腐蚀与清洗 将硬化的标本块放入盐酸中,室温下腐蚀一周左右,用蒸馏水流水缓缓冲洗,洗去腐败组织,直至满意,水流量要以不损伤铸型标本为度.1.6 显微解剖与剥制铸型 将腐蚀好的血管
1 Materials and methods 1.1 The use of fresh cadaver head, non-central nervous system death, usually within 7 hours after the death perfusion.1.2Prepolymerization of the mold agent with 9 parts methyl methacrylate (Methylmethacrylate) mixed with 1 part of acrylic acid (Methyl-crylate toughening agent), and add 6% benzoyl peroxide (Benjoylperoxide initiator) in the above two liquids.The mixture was put into a conical flask, the water bath was heated to 78 ~ 80 ℃. When the bath viscosity is 25 ~ 30% water-based glycerol-like concentration, yellow and transparent when the color is complete prepolymerization, rapid cooling. Monomer containing hydroquinone and other inhibitors, pre-polymerization with 1 ~ 2 % NaOH to remove the polymerization inhibitor, and then washed with distilled water until neutral, with molecular sieve (activated carbon) to the water .1.3 resin perfusion fresh cadaver removed, the bilateral internal carotid artery and vertebral artery cannulation with Rinse with saline until the venous outflows clear water. Fix the vascular endothelium with 30-40ml of 0.5% glutaraldehyde first, and then introduce the monomer into the pre-polymer by adding a small amount of Dimethylanilin (Dimethylanilin) Syringe injection, moderate pressure, until the internal jugular vein and vertebral artery out of the prepolymer, and then placed in the cadaver 5 0 ~ 60 ℃ immersion in water for several hours, can maintain the original shape, but also contribute to the hardening of the resin .1.4 material to be cast hard after craniotomy brain (usually about 2 to 3 days.) According to the site to be observed by the first material, size Depending on the requirements .1.5 Corrosion and cleaning of tissue Hardened specimen block into hydrochloric acid, corrosion at room temperature for about a week, slowly rinse with distilled water to wash away corrupt tissue, until satisfied, the water flow to not damage the mold Specimens are degrees .1.6 Microdissection and stripping Castings will erode good blood vessels