,Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis indu

• 作 者: Ming-qing GAO Shen-bo GUO Xue-hong CHEN Wei DU Chun-bo WANG
• 机 构: Medical College, Qingdao University, Qingdao 266021, China
• 出 处: 中国药理学报（英文版）
• 发表日期: 2007年7期
• 关键词: polypeptide from Chlamys farreri anti-oxidant HaCaT apoptosis UV irradiation

Aim: To investigate the mechanism of polypeptide from Chlamysfarreri (PCF) protecting HaCaT cells from apoptosis induced by UVA plus UVB in vitro. Methods: An apoptotic model of UV irradiation-induced HaCaT cells was established. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, agarose gel electrophoresis, biochemical methods, and Weste blotting were employed in the study. Results: PCF inhibited the UV irradiation-induced apoptosis of HaCaT cells. PCF strongly reduced the intracellular reactive oxygen species level, enhanced activities of superoxide dismutase and glutathione per-oxidase and increased the total anti-oxidative capacity in HaCaT cells following UV irradiation. Furthermore, we found that PCF could inhibit the phosphorylation of c-Jun amino-terminal kinase and the activity of caspase-3 in a concentration-dependent manner. Conclusion: PCF protected HaCaT cells from apoptosis in-duced by UVA plus UVB, mainly through decreasing the intracellular ROS level and increasing the activities of anti-oxidative enzymes to block the ROS-JNK-caspase-3-apoptosis signaling pathway.