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Using the method of dual-wavelength measurement of platelet [Ca~(2+)]_i and Fnra-2 as the Ca~(2+) fluorophore probe, we measured the effect of acidic Mu copolysaccharide front Sticopus Japonicus Selenka (SJAMP) on platelet [Ca~(2+)]_i.The results showed that the most significant increase in platelets [Ca~(2+)]_i was seen when the concentration of SJAMP wits 100μg/ml and the elevation of normal platelet [Ca~(2+)]_i was 93.96±10.24 nmol/L (n=10). In the presence of extracellular Ca~(2+)(1 mmol/L), the magnitude of platelet [Ca~(2+)]_i response to SJAMP was increased and the [Ca~(2+)]_i could reach 116.72±10.66 nmol/L (n=10). On the other hand, the magnitude of increased platelet [Ca~(2+)]_i induced by SJAMP was smaller and the duration of [Ca~(2+)]_i reaching the highest level was longer when compared with other platelet aggregation agents. In the mean time, if platelets were first incubated with cyclooxygenase inhibitor, the rise of [Ca~(2+)]_i evoked by SJAMP was inhibited. The results indicated that
Using the method of dual-wavelength measurement of platelet [Ca ~ (2 +)] _i and Fnra-2 as the Ca ~ (2+) fluorophore probe, we measured the effect of acidic Mu copolysaccharide front Sticopus Japonicus Selenka The results showed that the most significant increase in platelets [Ca ~ (2 +)] _ i was seen when the concentration of SJAMP wits 100 μg / ml and the elevation of normal platelet [Ca ~ (2 +)] _ i was 93.96 ± 10.24 nmol / L (n = 10). The presence of extracellular Ca 2+ (1 mmol / L), the magnitude of platelet [Ca 2+] response to SJAMP was increased and the [Ca ~ (2 +)] i could reach 116.72 ± 10.66 nmol / L (n = 10). On the other hand, the magnitude of increased platelet [Ca ~ (2 +)] _i induced by SJAMP was smaller and the duration of [Ca ~ (2 +)] _ i reaching the highest level was longer when compared with other platelet aggregation agents. The same time, if platelets were first incubated with cyclooxygenase inhibitor, the rise of [Ca ~ (2 +)] _ i evoked by SJAMP was inhibited. The resu lts indicated that