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对数生长期的人脐静脉血管内皮细胞(Human umbilical vein endothelial cells,HUVECs)分3组进行不同处理后采用流式细胞仪检测细胞周期分布;Annexin V/PI双染法测定细胞凋亡率;Western blot检测Bcl-2蛋白的表达情况;RT-PCR法检测Bcl-2 mRNA的表达情况。结果显示,硫酸镁可以在X-射线照射24 h后改善HUVECs细胞G2/M期阻滞;在辐照后24、48、72 h可以提高细胞对X-射线的辐射敏感性,并增加细胞凋亡率(t=4.24、7.19、3.20,p<0.05);在辐照后48、72 h能明显抑制抗凋亡蛋白Bcl-2蛋白的表达;照后24、48、72 h的Bcl-2 mRNA表达量减少(t=3.034、6.182、3.957,p<0.05)。结果提示,硫酸镁可以缓解X-射线照射后细胞G2/M期阻滞,增加照射后HUVECs的细胞凋亡率,降低X-射线诱导的抗凋亡蛋白Bcl-2的表达。
The logarithmic growth phase of human umbilical vein endothelial cells (HUVECs) were divided into 3 groups. The cell cycle distribution was detected by flow cytometry. The apoptosis rate was determined by Annexin V / PI double staining. The expression of Bcl-2 protein was detected by Western blot. The expression of Bcl-2 mRNA was detected by RT-PCR. The results showed that magnesium sulfate could improve G2 / M phase arrest of HUVECs 24 h after X-ray irradiation. Radiation sensitivity of X-ray to cells at 24, 48 and 72 h after irradiation increased, (T = 4.24,7.19,3.20, p <0.05). The expression of Bcl-2 protein at 48 and 72 h after irradiation significantly inhibited the expression of Bcl-2 protein at 24 h, 48 h and 72 h mRNA expression decreased (t = 3.034,6.182,3.957, p <0.05). The results suggest that magnesium sulfate can alleviate G2 / M arrest after X-ray irradiation, increase the apoptosis rate of HUVECs after irradiation, and decrease the expression of Bcl-2 induced by X-ray.