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目的:探讨血管内皮生长因子(VEGF)+936C/T、-634G/C两个位点单核苷酸多态性与子痫前期易感性的相关性。方法:采用双脱氧链末端终止法进行DNA测序,对2012年1月至2013年1月在本医院住院分娩的58例子痫前期,70例正常妊娠女性的VEGF+936C/T、-634G/C单核苷酸位点进行基因分析,比较各组基因型和等位基因频率分布有无差异。用优势比(OR)计算VEGF+936C/T基因在子痫前期患病中的相对危险度,比较两组中两个位点不同等位基因孕妇的血压、血肌酐、24 h尿蛋白定量等临床指标。结果:子痫前期组VEGF+936CT、TT基因型、T等位基因高于正常对照组,VEGF+936CC基因型、C等位基因低于正常对照组,差异有统计学意义(P<0.05);VEGF-634G/C位点的基因型、等位基因频率分布在子痫前期组及正常对照组孕妇中比较,差异均无统计学意义(P>0.05);正常对照组、子痫前期组人群中携带VEGF+936CT、TT基因型组发生子痫前期的危险性是CC基因型组的2.557倍;子痫前期组+936C/T位点CT+TT基因型孕妇与CC基因型孕妇的各临床指标比较,-634G/C位点GC+GG基因型孕妇与CC基因型孕妇各临床指标比较差异均有统计学意义(P<0.05);而对照组+936C/T位点CT+TT基因型孕妇与CC基因型孕妇的各临床指标比较,-634G/C位点GC+GG基因型孕妇与CC基因型孕妇各临床指标比较,差异均无统计学意义(P<0.05)。结论:携带VEGF+936CT、TT基因型者患子痫前期的危险性增加,-634G/C位点的G等位基因孕妇比携带C等位基因者更具有子痫前期遗传易感性,提示VEGF单核苷酸多态性可能与子痫前期的易感性相关。
Objective: To investigate the relationship between the single nucleotide polymorphisms of vascular endothelial growth factor (VEGF) + 936C / T and -634G / C two sites and the susceptibility to preeclampsia. Methods: DNA sequencing was performed by the dideoxy chain termination method. The VEGF + 936C / T and -634G / C in 58 preeclampsia and 70 normal pregnant women hospitalized in our hospital from January 2012 to January 2013 were analyzed. Single nucleotide analysis of genetic loci, genotypes and alleles in each group were compared whether the frequency distribution. The odds ratio (OR) was used to calculate the relative risk of VEGF + 936C / T gene in preeclampsia, and the blood pressure, serum creatinine, 24 h urinary protein, etc. of pregnant women with different alleles at two sites in two groups were compared Clinical indicators. Results: The levels of VEGF + 936CT, TT genotype and T allele in preeclampsia group were significantly higher than those in normal control group (P <0.05). The genotypes of VEGF + 936CC and C allele were lower than those in normal control group (P <0.05) There was no significant difference in the genotype and allele frequencies of VEGF-634G / C locus among pregnant women with preeclampsia and normal controls (P> 0.05). There was no significant difference between the normal control group, preeclampsia group The risk of preeclampsia in the TT genotype group was 2.557 times that of the CC genotype group in the population with VEGF + 936CT. In the preeclampsia + 936C / T genotype, both the CT + TT genotype and the CC genotype Compared with the clinical indicators, there were significant differences in the clinical indicators of pregnant women with CC genotype (-634G / C genotype GC + GG genotypes and CC genotypes pregnant women (P <0.05); while the control group +936C / T CT + TT gene Type of pregnant women and CC genotypes of pregnant women compared with the clinical indicators, -634G / C locus GC + GG genotype pregnant women and CC genotypes of pregnant women compared with the clinical indicators, the difference was not statistically significant (P <0.05). CONCLUSIONS: The risk of preeclampsia is increased in patients with VEGF + 936CT and TT genotypes, but the G allele at -634G / C is more susceptible to preeclampsia than those with C allele, suggesting that VEGF Single nucleotide polymorphisms may be associated with susceptibility to preeclampsia.