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目的探讨外周血T淋巴细胞、NK细胞分群在类风湿关节炎(RA)中的意义及与IL-6水平的相关情况。方法流式细胞仪法检测外周血淋巴细胞,记录20例RA患者和15例正常人的类风湿因子、血沉、抗核抗体(ANA)及免疫球蛋白、补体C3水平,ELISA法检测血清IL-6水平,并记录RA患者临床资料。结果活动期RA患者外周血CD3+T细胞计数与对照组分别为(68.8±7.8)、(62.3±4.2),活动期RA患者外周血CD3+T细胞计数轻度增高,但无统计学差异;RA患者外周血NK细胞(CD3-CD56+NK)计数与对照组分别为(9.8±1.8)、(12.3±2.0),RA患者明显低于对照组,P<0.05;活动期RA患者IL-6水平高于对照组,分别为(13.8±4.4)ng/L、(4.8+3.0)ng/L,有统计学意义。结论IL-6水平增高、CD3+T细胞和NK细胞表达失衡,其各自免疫调节作用在RA中可能体现不同的效应作用,RA的发病机制中三者间可能具有相互调节的免疫机制。
Objective To investigate the significance of peripheral blood T lymphocytes and natural killer (NK) cell population in rheumatoid arthritis (RA) and their correlation with IL-6 levels. Methods Peripheral blood lymphocytes were detected by flow cytometry. Rheumatoid factor, erythrocyte sedimentation rate, antinuclear antibody (ANA), immunoglobulin and complement C3 were detected in 20 patients with RA and 15 normal controls. Serum IL- 6 levels, and record the clinical data of RA patients. Results The counts of CD3 + T cells in peripheral blood of patients with active RA were (68.8 ± 7.8) and (62.3 ± 4.2), respectively. The counts of CD3 + T cells in patients with active RA were slightly increased, but there was no significant difference. The counts of NK cells (CD3-CD56 + NK) in peripheral blood of patients with RA were (9.8 ± 1.8) and (12.3 ± 2.0), respectively, significantly lower in patients with RA than those in controls (13.8 ± 4.4) ng / L and (4.8 + 3.0) ng / L, respectively, which were statistically significant. Conclusion The expression of IL-6 is increased, and the expression of CD3 + T cells and NK cells are imbalanced. Their respective immunoregulatory effects may have different effects in RA. The pathogenesis of RA may have a mutually regulated immune mechanism.