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黄曲霉毒素B_1和B_2经过量的氢硼化钠(NaBH_4)处理后,定量地还原成相应的三羟基衍生物(分别简称RB_1和RB_2),所产生的荧光强度可在紫外光下与标准黄曲霉毒素的衍生物比较定量。反应快速、简单。按常法制备黄曲霉毒素B_1和B_2的氯仿提取液。取提取液一定量(含B_11微克,B_2 0.5微克)于2只管形瓶中,置蒸气浴上于氮气流下蒸干。用100微升氯仿重新溶解。每瓶各加20微升NaBH_4溶液(1毫克/毫升甲醇。临用时新配),加盖,用力振摇,室温下放置10分钟,于氮气流下蒸干。
Aflatoxins B_1 and B_2 were quantitatively reduced to the corresponding trihydroxy derivatives (RB_1 and RB_2, respectively) after treatment with an amount of sodium borohydride (NaBH_4). The resulting fluorescence intensity was comparable to that of standard yellow Aspergillus toxin derivatives are more quantitative. The reaction is quick and easy The chloroform extract of aflatoxins B_1 and B_2 was prepared according to the usual method. Take a certain amount of extract (containing B_11 micrograms, B_2 0.5 micrograms) in two vials, steam bath on a nitrogen stream and evaporated to dryness. Re-dissolve with 100 μl chloroform. Add 20 μl of NaBH 4 solution to each bottle (1 mg / ml methanol). Cover and shake vigorously for 10 minutes at room temperature and evaporate under nitrogen flow.