论文部分内容阅读
用盆栽试验研究了不同含量Se(Ⅳ)和Se(Ⅵ)对小白菜生长和生理效应的影响.结果表明,不同含量Se(Ⅳ)和Se(Ⅵ)对小白菜体生长和生理代谢的影响不尽相同.低含量硒(Se(Ⅳ)<10.0mg·kg-1、Se(Ⅵ)<5.0mg·kg-1)能够促进小白菜的生长,使其根变粗,诱导脯氨酸的产生,阻止丙二醛(MDA)的合成,增强白菜的抗逆性,使其生物量明显提高;高含量硒会抑制小白菜的生长,导致植物体内脯氨酸合成下降,MDA含量增加,叶及根系生长受到抑制,地上和地下部干重显著急速下降(p<0.01).小白菜根长、叶面积等生长指标均随Se(Ⅳ)和Se(Ⅵ)含量的增大而显著下降(p<0.01);抑制小白菜根系生长的Se(Ⅳ)含量为60mg·kg-1,明显高于Se(Ⅵ)含量(20.0mg·kg-1).相同硒含量下,Se(Ⅳ)处理的小白菜地上部干重为Se(Ⅵ)的1.1～3.0倍,说明本试验条件下Se(Ⅵ)的生物有效性和毒性均大于Se(Ⅳ).Se(Ⅵ)处理小白菜地上部分的硒含量显著高于地下部分,而Se(Ⅳ)处理小白菜地下部分的硒含量显著高于地上部分,小白菜地上和地下部分对Se(Ⅵ)的吸收量均显著高于Se(Ⅳ).综合生长和生理因子分析表明,地上部分生物量是反映Se(Ⅳ)及Se(Ⅵ)处理土壤上小白菜生长状况的理想指标.
Pot experiments were conducted to study the effects of different contents of Se (Ⅳ) and Se (Ⅵ) on the growth and physiological characteristics of Brassica chinensis. The results showed that the effects of different contents of Se (Ⅳ) and Se (Ⅵ) (Se (Ⅳ) <10.0mg · kg-1, Se (Ⅵ) <5.0mg · kg-1) could promote the growth of Brassica chinensis, make its root thicker and induce proline Produce and prevent the synthesis of malondialdehyde (MDA), and enhance the resistance of Chinese cabbage, so as to significantly increase the biomass of the Chinese cabbage. High content of selenium can inhibit the growth of the Chinese cabbage and lead to the decrease of the proline synthesis and MDA content in the plant, (P <0.01). The growth indexes of root length and leaf area of Chinese cabbage decreased significantly with the increase of Se (Ⅳ) and Se (Ⅵ) content (P <0.01) (P <0.01). The content of Se (Ⅳ) in the root system of Chinese cabbage was 60 mg · kg-1, which was significantly higher than that of Se (Ⅵ) (P <0.01), indicating that the bioavailability and toxicity of Se (Ⅵ) were higher than that of Se (Ⅳ) under the conditions of this experiment.Se (Ⅵ) treatment of the aerial part of Chinese cabbage Selenium content significantly Se (Ⅳ) treatment showed that the selenium content of the underground part of Chinese cabbage was significantly higher than that of the aboveground part, and Se (Ⅵ) aboveground and underground part of Chinese cabbage was significantly higher than Se (Ⅳ) Factor analysis indicated that aboveground biomass was an ideal indicator to reflect the growth status of Chinese cabbage on Se (Ⅳ) and Se (Ⅵ) treatments.