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2012年1月,广东省经历了2次低温天气。本研究分别于2次降温后(3~10℃)和正常温度下(>10℃)采集广州和肇庆2片试验林的非洲桃花心木叶片,测定相对电导率(REC)、丙二醛(MDA)含量、脯氨酸(Pro)含量、超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性5个指标,分析了27个种源对寒害的生理响应模式。结果表明,2次降温均显著提高了非洲桃花心木的REC、Pro含量、SOD和POD活性。相比首次降温,第2次降温后,Pro含量、SOD和POD活性进一步提高,而REC则有所降低,表明植株在2次降温后进一步启动了自身的保护系统。该研究应用耐寒隶属函数值对27个种源进行综合评价,初步选出来自马里(种源10#,20#,22#)和布基纳法索(种源30#,31#,32#)的6个耐寒表现优异的种源。
In January 2012, Guangdong experienced two cold weather. In this study, African mahogany leaves from two experimental forests in Guangzhou and Zhaoqing were collected after 2 times of cooling (3 ~ 10 ℃) and normal temperature (> 10 ℃) respectively. The relative conductivity (REC), malondialdehyde MDA content, Pro content, SOD activity and POD activity were analyzed. The physiological responses of 27 provenances to cold stress were analyzed. The results showed that twice cooling reduced the content of REC, Pro, SOD and POD of mahogany significantly. Compared with the first cooling, the contents of Pro, SOD and POD increased further after the second cooling, while REC decreased, which indicated that the plant started its own protection system after the second cooling. In this study, we evaluated the 27 provenances using cold-tolerant membership values, and selected from Mali (provenances 10 #, 20 #, 22 #) and Burkina Faso (provenances 30 #, 31 #, 32 # The 6 hard-working outstanding provenance.