论文部分内容阅读
目的探讨外周血淋巴细胞表面标志物CD69在新生儿感染性疾病诊断中的价值。方法选取2007年3月-2008年12月在新生儿科(包括NICU)住院的患儿82例,分为重症感染组(42例)、轻症感染组(18例)和非感染组(22例)。应用流式细胞仪测定各组患儿外周血淋巴细胞CD69表达水平。应用SPSS 10.0软件进行统计学分析。结果非感染组、轻症感染组、重症组患儿外周血淋巴细胞CD69表达百分比分别为(1.13±0.69)%、(2.47±1.03)%、(2.16±1.24)%。轻症感染组患儿外周血淋巴细胞CD69表达水平显著高于非感染组(P=0.003),重症感染组患儿外周血淋巴细胞CD69表达水平显著高于非感染组(P=0.017),轻症感染组与重症感染组比较差异无统计学意义(P=0.546)。接受者操作特性曲线(ROC)计算得出CD69取临界值为2.19%,诊断新生儿感染的敏感度为42%,特异度为100%;CD69取临界值为1.29%,敏感度为83%,特异度为56%;CD69取临界值为0.99%,敏感度为92%,特异度为56%。结论新生儿感染时外周血淋巴细胞CD69表达水平明显升高,可作为早期诊断新生儿感染的实验室依据之一。
Objective To investigate the value of peripheral blood lymphocyte surface marker CD69 in the diagnosis of neonatal infectious diseases. Methods Totally 82 children hospitalized in neonatology (including NICU) from March 2007 to December 2008 were divided into severe infection group (42 cases), mild infection group (18 cases) and non-infection group (22 cases ). The expression of CD69 in peripheral blood lymphocytes of children in each group was measured by flow cytometry. SPSS 10.0 software was used for statistical analysis. Results The percentage of CD69 expression in non-infected group, mild infection group and severe group were (1.13 ± 0.69)%, (2.47 ± 1.03)%, (2.16 ± 1.24)%, respectively. CD69 expression in peripheral blood lymphocytes in children with mild infection was significantly higher than that in non-infected (P = 0.003), CD69 expression in peripheral blood lymphocytes in severe infection was significantly higher than that in non-infected (P = 0.017), mild There was no significant difference between severe infection group and severe infection group (P = 0.546). The receiver operating characteristic curve (ROC) calculated CD69 as the cut-off value of 2.19%, diagnosis of neonatal infection was 42% sensitivity and specificity of 100%; CD69 to the critical value of 1.29%, a sensitivity of 83% The specificity was 56%; CD69 was 0.99% with a sensitivity of 92% and a specificity of 56%. Conclusion The expression of CD69 in peripheral blood lymphocytes is significantly increased during neonatal infection and may serve as a laboratory basis for early diagnosis of neonatal infection.