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目的研究血标本离体后的预处理及保存方式对HCV-RNA检测结果的影响。方法取20份丙肝患者未分离及已分离的血浆标本,于不同时间及不同温度保存后,采用RT-PCR法,检测标本中HCV-RNA含量。结果抽血后1h内立即分离血浆的标本,分别保存于室温、4℃及-20℃,并于1 d及1周后分别检测HCV-RNA含量,结果取对数后进行比较,差异无统计学意义。抽血后不分离血浆的标本,保存于4℃,于24 h后进行检测,与已分离血浆,同样保存条件的标本检测结果取对数进行比较,结果差异有统计学意义。结论进行HCV-RNA检测时,为保证检测结果准确,血标本离体后应立即分离血清或血浆进行保存。
Objective To study the effects of pretreatment and preservation of blood samples on the detection of HCV-RNA in vitro. Methods Twenty non-isolated and isolated plasma samples from patients with hepatitis C were collected at different times and at different temperatures. The contents of HCV-RNA in the samples were determined by RT-PCR. Results Plasma samples were immediately separated within 1h after blood sampling and were stored at room temperature, 4 ℃ and -20 ℃ respectively. The contents of HCV-RNA were detected at 1 and 1 week respectively. The results were logarithmically and the difference was not statistically significant Significance of learning. Blood samples were not separated after blood was collected and stored at 4 ° C. After 24 h, the samples were compared with the logarithm of the test results of the separated plasma and the same storage conditions, and the difference was statistically significant. Conclusions When HCV-RNA test is performed, in order to ensure accurate test results, blood samples should be separated from serum and plasma immediately after isolation.