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研究L-茶氨酸对肝细胞损伤的保护作用及其机制。利用H2O2诱导的L02肝细胞损伤模型,分别用MTT法检测细胞存活率、测定LDH、流式细胞术检测细胞凋亡率、Western blot法检测Caspase-3和PARP蛋白表达及Bax/Bcl-2比值的变化,评价L-茶氨酸是否能保护H2O2诱导的肝细胞损伤。结果表明,L-茶氨酸能提高H2O2损伤的L02细胞存活率,减少LDH的渗漏,降低肝细胞凋亡,且L-茶氨酸通过抑制Caspase-3的激活和PARP的切割及Bax/Bcl-2比值的升高而发挥抗凋亡的作用。L-茶氨酸对肝细胞损伤有一定的治疗和保护作用。
To study the protective effect of L-theanine on hepatocyte injury and its mechanism. H2O2-induced L02 hepatocyte injury model was used to detect cell viability by MTT assay, LDH was determined by flow cytometry, apoptosis rate was detected by flow cytometry, Caspase-3 and PARP protein expression and Bax / Bcl-2 ratio To evaluate whether L-theanine protects hepatocytes from H2O2-induced injury. The results showed that L-theanine could increase the survival rate of L02 cells damaged by H2O2, reduce the leakage of LDH and reduce the apoptosis of hepatocytes. L-theanine could inhibit the activation of Caspase-3 and the cleavage of PARP and Bax / Bcl-2 ratio and play an anti-apoptotic effect. L-Theanine has certain therapeutic and protective effects on liver cell injury.