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目的 探讨烟雾与弹性蛋白酶联合应用对兔阻塞性肺气肿形成过程中肺功能和组织结构的影响。方法 将 48只新西兰大白兔随机分为吸烟组、注酶组、联合组及对照组 ,分别给予香烟熏吸、气管内注入猪胰弹性蛋白酶或生理盐水。 7周后进行肺功能、动脉血气和肺组织学检查。结果 注酶组和联合组的潮气量 (VT)、0 3s用力呼气容积 (FEV0 3 )、FEV0 3 /FVC(用力肺活量 )、PaO2 、平均肺泡数 (Na)和肺泡隔面密度 (Ds)减少 ,功能残气量 (FRC)、PaCO2 、肺总容积 (TLC)和肺泡直径 (Da)增加 ,与对照组比较 ,差异有显著性 (P <0 0 5 ,P <0 0 1) ,而吸烟组变化不明显 (P >0 0 5 )。联合组肉眼及光镜下可见有明显阻塞性肺气肿表现 ,注酶组有轻度肺气肿变化 ,吸烟组无明显变化。结论 烟雾可强化弹性蛋白酶的作用 ,二者联合应用可加快兔肺气肿的诱导过程
Objective To investigate the effects of combination of smoke and elastase on pulmonary function and tissue structure during the development of obstructive pulmonary emphysema in rabbits. Methods 48 New Zealand white rabbits were randomly divided into smoking group, enzyme group, combination group and control group, respectively, smoked cigarettes were given intratracheal instillation of porcine pancreatic elastase or normal saline. Pulmonary function, arterial blood gas and lung histology were taken after 7 weeks. Results VT, FEV0 3, FEV0 3 / FVC, PaO2, mean alveolar number (Al) and alveolar septum density (Ds) (P <0.05, P <0.01), and smoking, smoking, smoking and smoking were significantly different from those in the control group Group change was not obvious (P> 0.05). The combined group of naked eye and light microscope showed obvious obstructive pulmonary emphysema performance, injection group mild emphysema changes, smoking group no significant change. Conclusion The smoke can strengthen the role of elastase, the combination of the two can accelerate the induction of rabbit emphysema