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目的:研究氯丙嗪(Chl)、维拉帕米和阿斯匹林对撤除苯巴比妥钠(Phe)引起的小鼠肝细胞凋亡有无抑制作用及其时效关系。方法:以肝DNA含量、肝/体重比、DNA片段含量、DNA电泳、原位DNA末端标记试验(TUNEL)及形态学改变为指标,研究Phe 75mg·kg~(-1)·d~(-1)ip诱导小鼠肝细胞增殖及撤药后增殖肝回落的动态过程,并观察Chl25mg·kg~(-1)、维拉帕米50mg·kg~(-1)或阿司匹林60mg·kg~(-1)ip对小鼠肝细胞凋亡的影响及其时程效应。结果:Phe诱导小鼠肝细胞增殖及停药后增殖肝的回落由增殖期、平台期、快速回落期和缓慢回落期四个时相组成,快速回落期是一个典型的凋亡过程,其早期能被钙调蛋白拮抗剂Chl阻断。结论:钙调蛋白在撤除Phe引起的小鼠肝细胞凋亡中起重要作用。
AIM: To investigate the inhibitory effect of chlorpromazine (Chl), verapamil and aspirin on hepatocellular apoptosis in mice induced by phenobarbital (Phe) withdrawal and its time-effect relationship. Methods: The changes of liver DNA content, liver / body weight ratio, DNA fragment content, DNA electrophoresis, TUNEL and morphological changes were used as indexes to study the effect of Phe 75 mg · kg -1 · d ~ 1) ip induced the proliferation of mice hepatocytes and the dynamic process of proliferation of liver after withdrawal, and observed Chl25mg · kg -1, verapamil 50mg · kg -1 or aspirin 60mg · kg ~ (-1) -1) ip on mouse hepatocyte apoptosis and its time course effect. Results: Phe-induced hepatocyte proliferation and withdrawal of the proliferating liver decreased from the four phases of proliferative phase, plateau phase, rapid decline phase and slow down phase. The rapid decline was a typical apoptotic process. The early stage Can be blocked by the calmodulin antagonist Chl. Conclusion: Calmodulin plays an important role in the removal of Phe-induced hepatocyte apoptosis in mice.