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作者等继1986年首次证实山东省恙虫病流行之后,于1987年9～11月间,在流行区进行了病原分离.用小鼠分别自小盾纤恙螨(Leptotrombidium(L) scutellarae)、黑线姬鼠(Apodemus agrarius)以及恙虫病患者血液分离出8株恙虫病立克次体,分离阳性率分别为10、25和50%.所有分离物感染小鼠的肝脾均有3～5倍的扩大,其中3株仅血清抗体阳性而小鼠不发病;5株可使小鼠有规律地发病,并可以从病鼠组织中查见立克次体颗粒,但毒力有区别,其中3株症状不典型、发病晚,有2株感染小鼠接种后5～12天发病,症状典型,7～15天部分小鼠死亡(25～75%).用酶标染色法进行血清学鉴定,结果表明.分离株与普氏立克次体(E株)、Q热立克次体(06株)和斑点热立克次体(246株)无任何交叉反应,而与恙虫病立克次体(Gilliam株)呈阳性反应.初步认为,自山东分离的恙虫病立克次体可能是与Gilliam同一血清型.但山东恙虫病为秋冬型.
After confirming the epidemic of scrub typhus in Shandong Province for the first time in 1986, the authors isolated the pathogen in endemic areas from September to November 1987. The mice were isolated from Leptotrombidium (L) scutellarae, Eight strains of Rickettsia tsutsugamushi were isolated from the blood of Apodemus agrarius and tsutsugamushi patients, and the positive rates were 10, 25 and 50%, respectively.All isolates were infected with 3 ~ 5 times of liver and spleen Of which 3 were seropositive only in mice but not in mice; 5 mice were able to get regular disease, and rickettsial granules could be found in diseased mice, but the virulence was different, among which 3 Strain symptoms atypical, late onset, 2 infected mice 5 to 12 days after inoculation onset, the typical symptoms, 7 to 15 days in some mice died (25 to 75%). Serological identification with enzyme-linked staining, The results showed that there was no cross-reaction between the isolate and Rickettsia przewalskii (E strain), Q fever rickettsiae (06 strain) and Spotted fever rickettsia (246 strains) (Gilliam strain) was positive.It is initially believed that Rickettsia tsutsugamushi isolated from Shandong may be the same serotype Gilliam.But Shandong tsutsugamushi disease is Winter type.