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目的探讨I-κB激酶ε(IKKε)在卵巢癌组织中的表达及与卵巢癌转移的关系。方法采用实时定量PCR(qRT-PCR)和Western blot法分别检测53例不同临床分期的卵巢癌组织和10例正常卵巢组织中IKKεmRNA和蛋白表达,并分析IKKε表达与卵巢癌转移的相关性。结果Ⅱ、Ⅲ、Ⅳ期卵巢癌组织中IKKεmRNA和蛋白表达高于正常卵巢组织和Ⅰ期卵巢癌组织(P<0.05),且IKKε表达随着卵巢癌临床分期升高而增加(P<0.05),但与卵巢癌的病理类型、组织学分型及年龄无明显相关性(P>0.05)。结论 IKKε表达与卵巢癌的临床分期密切相关,可望成为临床治疗卵巢癌的一个新靶点。
Objective To investigate the expression of I-κB kinase ε (IKKε) in ovarian cancer and its relationship with ovarian cancer metastasis. Methods The mRNA and protein expressions of IKKε in 53 cases of ovarian cancer and 10 cases of normal ovarian tissue were detected by real-time quantitative PCR (qRT-PCR) and Western blot respectively. The correlation between IKKε expression and ovarian cancer metastasis was analyzed. Results The expression of IKKεmRNA and protein in stage Ⅱ, Ⅲ and Ⅳ ovarian cancer was significantly higher than that in normal ovarian tissue and stage Ⅰ ovarian cancer (P <0.05), and the expression of IKKε was increased with the clinical stage of ovarian cancer (P <0.05) , But not with pathological type, histological type and age of ovarian cancer (P> 0.05). Conclusion The expression of IKKε is closely related to the clinical stage of ovarian cancer, which may be a new target for the clinical treatment of ovarian cancer.