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目的:探讨葡萄籽原花青素(grape seed proanthocyanidin extract,GSPE)对兔实验性心肌再灌注损伤(myocardial reperfusion injury,MRI)诱发的心肌细胞凋亡的保护作用。方法:建立兔在体心肌再灌注损伤模型,24只兔随机平分为4组:假手术对照组(A)、缺血再灌注模型组(B)分别用生理盐水灌胃,低剂量GSPE治疗组(C)用GSPE(100 mg/kg)灌胃,高剂量GSPE治疗组(D)用GSPE(200 mg/kg)灌胃,共喂养21 d。各组测定再灌注前后不同时间血清心肌肌酸激酶同工酶MB(CK-MB)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量,对照观察各组兔心肌超微结构变化及心肌细胞凋亡情况。结果:与B组相比,C、D组的血清CK-MB和MDA显著降低(P<0.05,P<0.01),血清SOD明显升高(P<0.01);病理分析显示,C、D组兔心肌细胞损伤明显减轻,心肌细胞凋亡的数目明显减少,D组凋亡指数(AI)明显降低(P<0.01)。结论:GSPE在减轻兔MRI的同时,对其引起的细胞凋亡有明显的保护作用,可能是其抗MRI的机制之一。
Objective: To investigate the protective effect of grape seed proanthocyanidin extract (GSPE) on cardiomyocyte apoptosis induced by myocardial reperfusion injury (MRI) in rabbits. METHODS: A rabbit model of myocardial reperfusion injury was established. 24 rabbits were randomly divided into 4 groups: sham operation control group (A) and ischemia reperfusion model group (B) were intragastrically administered with saline, and low-dose GSPE treatment group. (C) Gavage with GSPE (100 mg/kg) and high-dose GSPE treatment (D) were intragastrically administered with GSPE (200 mg/kg) for 21 days. Serum creatine kinase MB (MB-MB), superoxide dismutase (SOD), and malondialdehyde (MDA) levels were measured at different time points before and after reperfusion in each group. Changes in myocardial ultrastructure were observed in each group. And cardiomyocyte apoptosis. RESULTS: Compared with group B, serum CK-MB and MDA in group C and D were significantly lower (P<0.05, P<0.01), and serum SOD was significantly increased (P<0.01); pathological analysis showed that group C and D were included. Cardiomyocyte injury was significantly reduced in rabbits, and the number of myocardial cell apoptosis was significantly reduced. The apoptotic index (AI) of group D was significantly decreased (P<0.01). Conclusion: GSPE can significantly protect rabbits from MRI, and it may be one of the mechanisms of anti-MRI.