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利用一步电沉积法,以含有环氧基团的γ-环氧丙氧丙基三甲氧基硅烷(GPTMS)为无机杂化试剂和功能性交联试剂,通过壳聚糖(Chitosan,CS)、辣根过氧化物酶(HRP)和葡萄糖氧化酶(GOD)分子中-NH2与环氧基团的反应,在金电极表面原位制备交联型有机-无机生物杂化膜,得到共固定HRP和GOD的新型双酶生物传感器.实验证实了这种有机-无机生物杂化膜在不同酸、碱条件下都具有高的稳定性和耐用性,克服了CS酸溶的不足,从而扩大了其使用范围.在葡萄糖检测中,交联型双酶传感器HRP-GOD/GPTMS/CS/Au比无交联的双酶传感器HRP-GOD/CS/Au具有更高的灵敏度、更宽的线性范围,其线性范围为1μmol/L～351μmol/L,检测限为0.3μmol/L.
A one-step electrodeposition method was used to fabricate chitosan nanoparticles by chitosan (Chitosan, CS), spicy glycidyl trimethoxysilane (GPTMS) containing epoxy groups as inorganic hybrid reagent and functional crosslinking reagent (HRP) and glucose oxidase (GOD) molecules in the reaction of -NH2 with epoxy groups, in-situ preparation of cross-linked organic-inorganic hybrid membranes on the surface of gold electrode to obtain co-immobilized HRP and GOD biosensor.The experiments confirmed that this organic-inorganic hybrid membrane has high stability and durability under different acid and alkali conditions, overcomes the shortcomings of CS acid solution, thus expanding its use Range.In the glucose assay, HRP-GOD / GPTMS / CS / Au cross-linked double enzyme sensor has higher sensitivity and broader linear range than HRP-GOD / CS / Au without cross- The linear range was 1μmol / L ~ 351μmol / L, the detection limit was 0.3μmol / L.