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目的:建立高效液相色谱法联合蒸发光检测器测定枣仁安神胶囊中酸枣仁皂苷A含量的方法,为该制剂质量标准提供依据。方法:采用HPLC-ELSD方法,固定相为Agilent TC(2)-C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.2%醋酸(68:32),流速1.0 ml·min~(-1),柱温:30℃;ELSD检测雾化温度45℃,气体流速为1.6 L·min~(-1)。结果:酸枣仁皂苷A的线性范围为22.5～360.0μg·min~(-1),r=0.999 5,平均回收率为99.8%,RSD=1.06%(n=6)。结论:本法简便,灵敏度高,重复性好,可作为枣仁安神胶囊的质量控制方法。
OBJECTIVE: To establish a method for determination of Jujuboside A in Zaoren Anshen capsule by HPLC and evaporative light detector, and provide the basis for the quality standard of the preparation. Methods: The HPLC-ELSD method was used. The mobile phase was Agilent TC (2) -C 18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase consisted of methanol and 0.2% acetic acid min -1, the column temperature was 30 ℃, the atomization temperature was 45 ℃ and the gas flow rate was 1.6 L · min -1 by ELSD. Results: Jujuboside A had a linear range of 22.5-360.0 μg · min -1, r = 0.999 5, with an average recovery of 99.8% and a RSD of 1.06% (n = 6). Conclusion: This method is simple, sensitive, reproducible, and can be used as a quality control method for Zaoren Anshen capsules.