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目的探讨大鼠急性胰腺炎(AP)肺损伤时,肺组织损伤程度与组织巨噬细胞炎症蛋白-2(MIP-2)、肿瘤坏死因子-α(TNF-α)基因表达的关系。方法胰胆管逆行注射5%脱氧胆酸钠(DCA)复制大鼠AP模型,观察胰腺和肺组织病理学改变,使用RT-PCR法检测肺组织中MIP-2 mRNA、TNF-αmRNA的表达。结果AP 3 h、AP 6 h组胰腺和肺组织镜下病理评分明显高于相应对照组。AP 6 h组肺组织MIP-2 mRNA表达明显上调。胰腺病理评分与肺组织病理学评分、肺组织MIP-2基因表达呈正相关;肺组织MIP-2与TNF-α的基因表达之间也呈明显正相关。结论肺组织MIP-2基因表达上调,以及与TNF-α的相互诱生,可能是导致急性胰腺炎中性粒细胞在肺组织浸润和活化,进而导致肺损伤的重要机制之一。
Objective To investigate the relationship between lung injury and the expression of MIP-2 and TNF-α in rats with acute pancreatitis (AP) lung injury. Methods Pancreatic duct was injected retrogradely with 5% sodium deoxycholate (DCA) to observe the pathological changes of the pancreas and lung tissue. The expression of MIP-2 mRNA and TNF-α mRNA in lung tissue was detected by RT-PCR. Results The histopathological scores of pancreas and lung in AP 3 h and AP 6 h groups were significantly higher than those in corresponding control groups. AP 6 h lung tissue MIP-2 mRNA expression was significantly increased. There was a positive correlation between the pathological score of pancreatic tissue and the score of lung histopathology, and the expression of MIP-2 gene in lung tissue. The gene expression of MIP-2 and TNF-α in lung tissue also showed a positive correlation. Conclusions The up-regulation of MIP-2 gene expression and the interaction with TNF-α in lung tissue may be one of the important mechanisms leading to the infiltration and activation of acute pancreatitis in lung tissue and lung injury.