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目的 :探索99mTc标记抗人喉癌单克隆抗体 (McAb -Lc)的方法 ,观察99mTc-McAb -Lc在喉癌移植瘤模型体内的分布情况。方法 :应用改良Schwarz法进行99mTc标记McAb -Lc,用γ计数仪测定放射强度 ,计算肿瘤和非肿瘤组织的单位质量摄取放射性活度的百分比 (ID % g)和肿瘤与非肿瘤组织的放射性比值 (T NT)。结果 :改良Schwarz法制备的99mTc -McAb -Lc无毒、无菌、无热原 ,性质稳定 ,标记率在 90 %～ 95% ,抗体的活性在标记前后无明显改变。注射99mTc -McAb -Lc后 ,各时相肿瘤组织ID % g均显著高于注射99mTc-鼠IgG对照组 (P≤ 0 .0 0 3) ,注射99mTc -McAb -Lc后 2h和 6h ,肿瘤组织ID % g和肿瘤与血液的放射性比值 (T B)分别从 4.56± 0 .51、1 .0 3± 0 .1 7增加到 7.84± 1 .46和 1 .84± 0 .30 ,差异有高度显著性 (P≤ 0 .0 0 5)。结论 :改良Schwarz法制备的99mTc -McAb -Lc ,能较好地满足放射免疫显像 (RII)的要求 ,McAb -Lc的免疫导向作用可靠 ,注射后 6h ,99mTc -McAb -Lc在肿瘤组织中有相对较高的特异性浓聚。
Objective: To explore the method of 99mTc-labeled anti-human laryngeal carcinoma monoclonal antibody (McAb-Lc) in order to observe the distribution of 99mTc-McAb-Lc in the model of laryngeal carcinoma. Methods: 99mTc McAb-Lc was labeled with 99mTc by modified Schwarz assay. The radioactivity was measured by gamma counter. The radioactive radioactivity per unit mass of tumor and non-tumor tissues (ID% g) and radioactivity ratio of tumor and non-tumor tissue (T NT). Results: The 99mTc-McAb-Lc prepared by the modified Schwarz method was non-toxic, sterile and pyrogen free and stable in character. The labeling efficiency was 90% -95%. The activity of antibody was not changed before and after labeling. After injection of 99mTc-McAb-Lc, the ID% g of tumor tissue in each phase was significantly higher than that in 99mTc-mouse IgG injection group (P≤0.030), at 2h and 6h after injection of 99mTc -McAb- ID% g and tumor to blood radioactivity ratio (TB) increased from 4.56 ± 0.51,1.013 ± 0.17 to 7.84 ± 1.46 and 1.84 ± 0.30, the difference was highly significant Sex (P≤0.050). CONCLUSION: 99mTc-McAb-Lc prepared by modified Schwarz method can meet the requirements of radioimmunoimaging (RII). The immunosuppressive effect of McAb-Lc is reliable. At 6h after injection, 99mTc-McAb-Lc There is a relatively high concentration of specific.