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BACKGROUND: One of the major limitations of biliary stents is the stent occlusion, which is closely related to the overgrowth of bacteria. This study aimed to evaluate the feasibility of a novel silver-nanoparticle-coated polyurethane(Ag/PU) stent in bacterial cholangitis model in swine.METHODS: Ag/PU was designed by coating silver nanoparticles on polyurethane(PU) stent. Twenty-four healthy pigs with bacterial cholangitis using Ag/PU and PU stents were randomly divided into an Ag/PU stent group(n=12) and a PU stent group(n=12), respectively. The stents were inserted by standard endoscopic retrograde cholangiopancreatography. Laboratory assay was performed for white blood cell(WBC) count, alanine aminotransferase(ALT), interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) at baseline time, 8 hours, 1, 2, 3, and 7 days after stent placements. The segment of bile duct containing the stent was examined histologically ex vivo. Implanted biliary stents were examined by a scan electron microscope. The amount of silver release was also measured in vitro.RESULTS: The number of inflammatory cells and level of ALT, IL-1β and TNF-α were significantly lower in the Ag/PU stent group than in the PU stent group. Hyperplasia of the mucosa was more severe in the PU stent group than in the Ag/PU stent group. In contrast to the biofilm of bacteria on the PU stent, fewer bacteria adhered to the Ag/PU stent.CONCLUSIONS: PU biliary stents modified with silver nanoparticles are able to alleviate the inflammation of pigs with bacterial cholangitis. Silver-nanoparticle-coated stents are resistant to bacterial adhesion.
BACKGROUND: One of the major limitations of biliary stents is the stent occlusion, which is closely related to the overgrowth of bacteria. This study aimed to evaluate the feasibility of a novel silver-nanoparticle-coated polyurethane (Ag / PU) stent in bacterial cholangitis model in swine.METHODS: Ag / PU was designed by coating silver nanoparticles on polyurethane (PU) stent. Twenty-four healthy pigs with bacterial cholangitis using Ag / PU and PU stents were randomly divided into an Ag / PU stent group (n = The stents were inserted by standard endoscopic retrograde cholangiopancreatography. Laboratory assay was performed for white blood cell (WBC) count, alanine aminotransferase (ALT), interleukin-1β (IL- 1β), tumor necrosis factor-α (TNF-α) at baseline time, 8 hours, 1, 2, 3 and 7 days after stent placements. The segment of bile duct containing stent was examined histologically ex vivo were examined by a scan electron m The amount of silver release was also measured in vitro .RESULTS: The number of inflammatory cells and level of ALT, IL-1β and TNF-α were significantly lower in the Ag / PU stent group than in the PU stent group. Hyperplasia of the mucosa was more severe in the PU stent group than in the Ag / PU stent group. Less than bacteria adhered to the PU stent, fewer bacteria adhered to the Ag / PU stent. CONCLUSIONS: PU biliary stents modified with silver nanoparticles are able to alleviate the inflammation of pigs with bacterial cholangitis. Silver-nanoparticle-coated stents are resistant to bacterial adhesion.