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目的观察益气血补肝肾方对整合素ανβ3在早孕小鼠子宫内膜中表达的影响。方法采用免疫组织化学定位观察整合素ανβ3在早孕小鼠子宫内膜中的表达;采用实时荧光定量PCR在mRNA水平上检查整合素ανβ3在早孕小鼠子宫内膜中的表达。结果免疫组化结果显示,整合素ανβ3蛋白在妊娠早期小鼠表达于子宫内膜腔上皮、腺上皮细胞的细胞膜及胞浆中,呈棕黄色颗粒状。D1、D2表达最弱,D4表达明显增加,D5表达最强,D6表达减弱;实时荧光定量PCR结果显示,整合素ανβ3在中药组和对照组早孕小鼠子宫内膜中的表达随妊娠天数呈规律变化,在中药组的表达高于对照组,但差异无统计学意义。两组中D4和D5表达最强,与D1、D2、D6比较差异具有显著性(P<0.05),整合素αν在D5的表达显著高于D4(P<0.01)。结论益气血补肝肾方可改善子宫内膜的容受性,促进胚胎与子宫内膜发育的同步性,从而在胚胎着床过程中发挥重要作用。
Objective To observe the effect of Yiqi Xie Bugan She Fang on the expression of integrin ανβ3 in the endometrium of early pregnancy mice. Methods The expression of integrin ανβ3 in the endometrium of early pregnant mice was observed by immunohistochemical localization. The expression of integrin ανβ3 in the endometrium of early pregnant mice was detected by real-time fluorescence quantitative PCR. Results Immunohistochemical results showed that integrin αvβ3 protein was expressed in the endometrial cavity epithelial cells and cytoplasm of endometrial epithelial cells in the first trimester of pregnancy, showing brown granular. The expression of integrin ανβ3 in the endometrium of the pregnant mice in the traditional Chinese medicine group and the control group was significantly decreased with the number of gestational days Regular changes in the expression of traditional Chinese medicine group than the control group, but the difference was not statistically significant. The expression of D4 and D5 were the highest in both groups, but significantly different from D1, D2 and D6 (P <0.05). The expression of integrin αν in D5 was significantly higher than that in D4 (P <0.01). Conclusions Yiqi Xue Bugan decoction can improve endometrial receptivity and promote synchronicity of embryo and endometrium development, which play an important role in embryo implantation.