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目的利用基因芯片技术分析胎盘血管病变差异基因的表达,为深入探讨胎盘血管病变的发生机制提供新的线索。方法应用22K人类基因组寡核苷酸芯片,筛选了胎盘血管病变患者(实验组)和正常妊娠者(对照组)胎盘组织中的差异表达基因,应用定量聚合酶链反应(PCR)及蛋白印记分析(Western-blot)验证部分差异基因的表达。结果实验组与对照组比较,存在10个表达水平显著改变的基因,其中9个在胎盘血管病变中上调,1个下调。经验证部分差异基因的表达与芯片结果一致。结论胎盘血管病变是一种具有血管壁内皮细胞激活和免疫应答的炎性疾病。这些差异表达基因可能通过多种信号转导途径参与了内皮激活与炎症应答,并导致了胎盘血管病变的发生和发展。
Objective To analyze the expression of differentially expressed genes in placental vascular lesions by gene chip technology and provide new clues for further exploration of the pathogenesis of placental vascular lesions. Methods 22K human genome oligonucleotide microarray was used to screen differentially expressed genes in placental tissues of patients with placental vasculopathy (experimental group) and normal pregnancy (control group). Quantitative polymerase chain reaction (PCR) and Western blotting (Western-blot) to verify some of the differential gene expression. Results Compared with the control group, there were 10 genes with significant changes in the experimental group, of which 9 were up-regulated and 1 down-regulated in the placental vascular lesions. The difference between the validated and the differentially expressed genes is consistent with that of the chip. Conclusion Placental vascular disease is an inflammatory disease with endothelial cell activation and immune response in the blood vessel wall. These differentially expressed genes may participate in endothelial activation and inflammatory response through a variety of signal transduction pathways and lead to the occurrence and development of placental vascular lesions.