论文部分内容阅读
发展了一种以微流控芯片为平台的药物诱导细胞凋亡的新方法.选择HeLa细胞为对象,通过浓度梯度芯片形成稳定的药物浓度梯度,诱导HeLa细胞凋亡,利用荧光能量共振转移(fluorescence resonance energy transfer,FRET)成像系统进行实时监测,分析细胞对不同浓度化合物的毒性反应.结果表明,细胞在顺铂诱导下发生明显的起泡和皱缩,FRET比率值逐渐降低,在药物浓度梯度作用下,芯片每个通道内细胞呈现不同程度的凋亡.该方法实现了药物浓度梯度诱导细胞凋亡的实时监测和定量分析,为抗肿瘤药物评价和高通量药物筛选提供了新的手段.
A new method of drug-induced apoptosis using microfluidic chip as platform was developed.Apoptosis of HeLa cells was induced by the concentration gradient of HeLa cells and HeLa cells were induced by fluorescence gradient resonance energy transfer fluorescence resonance energy transfer (FRET) imaging system was used to monitor the cytotoxicity of cells to different concentrations of compounds.Results showed that the cells foamed and shrunk under the induction of cisplatin, and the FRET ratio decreased gradually, Under the action of gradient, the cells in each channel of the chip showed different degrees of apoptosis.The method realizes the real-time monitoring and quantitative analysis of apoptosis induced by drug concentration gradient and provides a new method for anti-tumor drug evaluation and high-throughput drug screening means.