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本文报告一种新方法,即将薄层层析 (TLC)与Ames方法结合起来,直接用指示菌在TLC板上检测诱变原。作者用市售硅胶或纤维素制成反相薄层层析板(revesed-phase TLC plates,10×10 cm),在无水酒精中展开后置室温下干燥5分钟,再于80℃干燥20分钟,然后进行灭菌处理。空白的TLC板上无诱变原存在,故只有自发回变。测试时将样品点在层析板上,在12×12 cm层析槽中于室温下展开,然后以254 nm和350 nm的荧光照射,使分离开的组分显荧光,或用常规方法在平行板上使之可见。
This article reports a new method of combining thin layer chromatography (TLC) with the Ames method and direct detection of mutagenic agents on TLC plates with indicator bacteria. The authors made revesed-phase TLC plates (10 × 10 cm) using commercially available silica gel or cellulose, developed in anhydrous alcohol, dried at room temperature for 5 minutes and then dried at 80 ° C for 20 minutes Minute, then sterilize. Blank TLC plate without mutagen exists, so only spontaneous change. The samples were spotted on a chromatographic plate, developed in a 12 x 12 cm chromatography cell at room temperature and then exposed to fluorescent light at 254 nm and 350 nm for fluorescence of the separated components or by conventional methods Make it visible on a parallel plate.