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采用L_9(3~4)正交实验法,研究了盐度(3、6、9)、光照强度[20、40、60μmol/(m~2·s)]和温度(10、20、30℃)3因素对半咸水小环藻(Cyclotella sp.)SHOU-B108生长及细胞中ARA和EPA含量的影响。结果表明:培养10 d后小环藻在盐度6、光照强度40μmol/(m~2·s)和温度30℃组具有最大生物量(干重)。盐度、光照强度和温度对小环藻细胞ARA和EPA含量的影响均有显著的交互作用,但温度是影响藻细胞ARA和EPA含量的主要因素。低的盐度(3)和高的温度(30℃)有利于小环藻细胞积累ARA;而高的光照强度[60μmol/(m~2·s)]则有利于细胞积累EPA。本研究表明环境因子对小环藻细胞中不同的脂肪酸会产生不同的诱导效应,通过调控小环藻的培养条件,能够获得特定营养物质含量丰富的饵料微藻。
The effects of salinity (3, 6, 9), light intensity [20, 40 and 60μmol / (m ~ 2 · s)] and temperature ) 3 on the growth of SHOU-B108 cells and the content of ARA and EPA in Cyclotella sp. The results showed that after 10 days of culture, Cyclotella concanavalina had the highest biomass (dry weight) at salinity 6, light intensity 40μmol / (m 2 · s) and temperature 30 ℃. Salinity, light intensity and temperature had significant interactions on the contents of ARA and EPA in Cyclotella, but temperature was the main factor affecting the content of ARA and EPA in algal cells. Low salinity (3) and high temperature (30 ° C) are favorable for the accumulation of ARA in the cells of the genus Cyclotella; high light intensity [60 μmol / (m 2 · s)] is beneficial for cells to accumulate EPA. This study shows that environmental factors have different inducing effects on different fatty acids in the cells of Cyclotella algae. By regulating the cultivation conditions of Cyclotella, we can obtain the food-rich microalgae with specific nutrient content.