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本研究利用组织培养技术对进行培养挽救,以为了提高育种成功率。试验结果表明:外植体的最佳消毒方法为75%酒精消毒30 s后在0.1%升汞处理8 min,幼胚的成活率为90%。随着外植体消毒时间增加,幼胚成活率先上升后下降。幼胚最佳萌发培养基为1/2MS+BA0.1 mg.L-1+NAA0.01 mg.L-1。最佳继代增殖培养基为MS+BA0.1 mg.L-1+NAA0.01 mg.L-1,增殖系数达到6.33。对不定芽增殖最有利条件为:pH值6.0、琼脂浓度6 g.L-1和蔗糖浓度30 g.L-1。最佳生根培养基为1/2MS+IBA0.2 mg.L-1,生根率为93.2%。
In this study, the use of tissue culture technology to save the rescue, in order to improve the success rate of breeding. The results showed that the optimal method for disinfection of explants was 75% alcohol for 30 s and 0.1% mercuric chloride for 8 min, the survival rate of immature embryos was 90%. As explant disinfection time increased, the survival rate of immature embryos increased first and then decreased. The optimal germination medium for immature embryos was 1 / 2MS + BA0.1 mg.L-1 + NAA0.01 mg.L-1. The best subculture medium was MS + BA0.1 mg.L-1 + NAA0.01 mg.L-1, the proliferation coefficient reached 6.33. The most favorable conditions for adventitious bud multiplication were: pH 6.0, agar concentration 6 g.L-1 and sucrose concentration 30 g.L-1. The best rooting medium was 1 / 2MS + IBA0.2 mg.L-1, the rooting rate was 93.2%.