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目的 探讨单核细胞表面 CD14、HL A- DR的表达在新生儿感染性疾病发生发展中的作用及与预后的关系。 方法 用双标记流式细胞术对新生儿感染患儿外周血单核细胞 CD14、HL A- DR的表面表达量进行检测 ,检测时间分别为诊断新生儿感染后的第 1、5～ 7天各测 1次 ,同时检测 C-反应蛋白及外周血白细胞。并以 34例非感染新生儿作为对照 ,并分析比较结果。 结果 感染组患儿入院即刻血清 CD14与对照组比较明显降低 (P<0 .0 0 1) ,血清 HL A- DR也显著低于对照组(P<0 .0 0 1) ;感染组患儿经治疗后血清 CD14水平逐渐升高 (P<0 .0 5 ) ,血清 HL A- DR水平较治疗前有所升高 (P<0 .0 1) ;而 2例死亡小儿随着病情的加重 CD14阳性细胞及其细胞中的 HL A- DR发生明显变化 ,呈明显降低趋势。 结论 新生儿感染患儿外周血单核细胞 CD14及 HL A- DR表面表达量均降低 ;且 CD14和 HL A- DR的总体表达水平与患儿病死率呈负相关
Objective To investigate the role of CD14, HL-DR expression on the mononuclear cells in the development of neonatal infectious diseases and its relationship with prognosis. Methods The surface expression levels of CD14 and HL-DR in peripheral blood mononuclear cells of neonates were detected by double-labeled flow cytometry. The detection time was respectively from day 1 to day 7 after diagnosis of neonatal infection Test 1, simultaneous detection of C-reactive protein and peripheral white blood cells. 34 cases of non-infected newborns as a control, and analysis of the results. Results Serum CD14 in infected patients was significantly lower than that in control group (P <0.01), serum HL-DR was also significantly lower than that in control group (P0.01) After treatment, serum CD14 levels gradually increased (P <0.05), serum HL-DR levels increased compared with those before treatment (P <0.01); while the two deaths of children with the aggravating disease HL-DR in CD14-positive cells and their cells changed significantly, showing a significant decrease trend. Conclusion The expression of CD14 and HL A-DR in peripheral blood mononuclear cells of neonates with neonatal infection were decreased. The overall expression levels of CD14 and HL-DR were negatively correlated with the mortality of children