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通过对HCCLM3-fLuc-GFP肝癌的进展和血管生成的多模态成像分析,对肝癌细胞在体内生长的适应过程和级数生长特性,分别进行了生物自发荧光成像(bioluminescent imaging,BLI)研究、激发荧光成像(fluorescent imaging,FMI)研究和小动物计算机断层成像(micro-computed tomography,Micro-CT)研究,对采集的成像数据进行定性定量分析,并与肿瘤的体积测量数据进行了相关性分析。结果表明,肿瘤细胞在接种后第4天细胞数量基本为原接种数量的一半,第7天体内的细胞基本与接种数量相等,7天内细胞基本完成了体内的适应过程,之后肝癌细胞进入了快速增殖期。BLI成像、FMI成像均与肿瘤体积的测量结果高度相关(R2=0.9263,R2=0.9068)。通过计算机断层成像(Micro-CT),对肿瘤的新生血管进行分析,并用可视化的手段对肿瘤的血管新生特性进行三维显示,Micro-CT成像结果验证了BLI、FMI成像揭示的肿瘤生长后期的非指数生长特性。
Through the analysis of the progress of HCCLM3-fLuc-GFP hepatocarcinoma and the multimodal imaging of angiogenesis, the bioluminescent imaging (BLI) of hepatocarcinoma cells in vitro and their growth characteristics were carried out. Fluorescence imaging (FMI) and micro-computed tomography (micro-computed tomography) studies of the animals were performed to qualitatively and quantitatively analyze the acquired imaging data and to correlate with the tumor volume measurement data . The results showed that the number of tumor cells on day 4 after inoculation was almost half of the original number of inoculated cells. On the 7th day, the number of cells in the body was almost equal to the number of inoculation, and the cells basically completed the process of in vivo adaptation within 7 days. Proliferation phase. Both BLI imaging and FMI imaging were highly correlated with tumor volume measurements (R2 = 0.9263, R2 = 0.9068). The neovascularization of tumor was analyzed by computerized tomography (Micro-CT), and the neovascularization characteristic of tumor was three-dimensionally visualized by means of visualization. The results of Micro-CT imaging confirmed that the non-tumorous growth phase of BLI and FMI Exponential growth characteristics.