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目的观察雷公藤对大鼠胰腺组织的病理损伤。方法参照长期毒性实验方法,将清洁级SD大鼠随机分成4组,即空白对照组、0.04 g/kg组、0.16 g/kg组和0.64 g/kg组。连续灌胃(ig)不同剂量雷公藤95%乙醇提取物3个月,取胰腺;0.64 g/kg组分别于用药30和90 d后及停药30 d后取胰腺组织,采用光学显微镜及透射电镜技术动态观察胰腺组织的病理结构变化,采用连续检测法测定各时间点血清淀粉酶含量。结果 0.04 g/kg组用药90 d后大鼠胰腺散在淋巴细胞增多,个别细胞胞浆空亮;0.16 g/kg组用药90 d后胰岛体积大,纤维增生;0.64 g/kg组用药90 d后,细胞胞浆空亮,间质纤维增生;0.64 g/kg组用药30 d后细胞水肿,小灶炎细胞浸润,线粒体肿胀;0.64 g/kg组停药30 d后胰腺内有红色分泌物潴留,纤维组织增生,伴淋巴细胞浸润,细胞线粒体肿胀、空泡化。0.64 g/kg组给药30和90 d和停药30 d后的血液淀粉酶水平无显著的上升或下降。结论雷公藤可引起大鼠胰腺组织的病理损伤,并随给药剂量加大和时间延长损伤加重且呈不可逆性。
Objective To observe the pathological injury of rat’s pancreas tissue by Tripterygium wilfordii. Methods According to the long-term toxicity test, clean-grade SD rats were randomly divided into 4 groups: blank control group, 0.04 g / kg group, 0.16 g / kg group and 0.64 g / kg group. Pancreatic gland was obtained by gavage with different doses of 95% ethanol extract of different doses of Tripterygium wilfordii for 3 months. The pancreatic tissues were harvested at 30 and 90 d after drug administration and 30 d after drug administration, respectively. Electron microscopy was used to observe the pathological changes of pancreatic tissue dynamically. Serum amylase content of each time point was determined by continuous detection. Results In the 0.04 g / kg group for 90 days, the number of scattered lymphocytes in the pancreas increased and the cytoplasm of individual cells became empty. After 90 days of treatment, the islet volume and fibrosis were increased in the group of 0.16 g / kg for 90 days , Cell cytoplasm empty space, interstitial fibrosis; 0.64 g / kg group after 30 d of cell edema, infiltration of small cells of inflammatory cells, mitochondria swelling; 0.64 g / kg group 30 d after withdrawal of the pancreatic retention of red secretions, Fibrous tissue proliferation, with lymphocyte infiltration, cell mitochondria swelling, vacuolization. There was no significant increase or decrease of blood amylase level at 0.64 g / kg for 30 and 90 d and 30 d after withdrawal. Conclusion Tripterygium wilfordii can cause pathological damage in rat pancreatic tissue, and with increasing dose and prolonged exposure, the injury is aggravated and irreversible.