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Previous studies suggest that serotonin(5-HT) might interact with brain-derived neurotrophic factor(BDNF) during the stress response.However,the relationship between 5-HT and BDNF expression under purely psychological stress is unclear.In this study,one hour before psychological stress exposure,the 5-HT1 A receptor agonist 8-OH-DPAT or antagonist MDL73005,or the 5-HT2 A receptor agonist DOI or antagonist ketanserin were administered to rats exposed to psychological stress.Immunohistochemistry and in situ hybridization revealed that after psychological stress,with the exception of the ventral tegmental area,BDNF protein and m RNA expression levels were higher in the 5-HT1 A and the 5-HT2 A receptor agonist groups compared with the solvent control no-stress or psychological stress group in the CA1 and CA3 of the hippocampus,prefrontal cortex,central amygdaloid nucleus,dorsomedial hypothalamic nucleus,dentate gyrus,shell of the nucleus accumbens and the midbrain periaqueductal gray.There was no significant difference between the two agonist groups.In contrast,after stress exposure,BDNF protein and m RNA expression levels were lower in the 5-HT1 A and 5-HT2 A receptor antagonist groups than in the solvent control non-stress group,with the exception of the ventral tegmental area.Our findings suggest that 5-HT regulates BDNF expression in a rat model of acute psychological stress.
Previous studies suggest that serotonin (5-HT) might interact with brain-derived neurotrophic factor (BDNF) during the stress response. However, the relationship between 5-HT and BDNF expression under purely psychological stress is unclear. before psychological stress exposure, the 5-HT1 A receptor agonist 8-OH-DPAT or antagonist MDL73005, or the 5-HT2 A receptor agonist DOI or antagonist ketanserin were administered to rats exposed to psychological stress. Immunohistochemistry and in situ hybridization revealed that after psychological stress, with the exception of the ventral tegmental area, BDNF protein and m RNA expression levels were higher in the 5-HT1 A and the 5-HT2 A receptor agonist groups compared with the solvent control no-stress or psychological stress group in the CA1 and CA3 of the hippocampus, prefrontal cortex, central amygdaloid nucleus, dorsomedial hypothalamic nucleus, dentate gyrus, shell of the nucleus accumbens and the midbrain periaqueductal gray .here was no significant difference between the two agonist groups. Contrast, after stress exposure, BDNF protein and m RNA expression levels were lower in the 5-HT1 A and 5-HT2 A receptor antagonist groups than in the solvent control non-stress group, with the exception of the ventral tegmental area. Our findings suggest that 5-HT regulates BDNF expression in a rat model of acute psychological stress.