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[目的]通过观察Rac1shRNA对卵巢癌细胞Skov3侵袭能力的影响。[方法]Rac1shRNA载体pGPU6/GFP/Rac1-524以脂质体LipofectamineTM 2000介导转染卵巢癌细胞株Skov3,Transwell侵袭实验检验转染细胞的迁移侵袭能力;扫描电镜观察转染Skov3细胞的超微结构变化。[结果]pGPU6/GFP/Rac1-524转染组细胞迁移距离最短,板状伪足消失,侵袭能力下降,与pGPU6/GFP/NC转染组和未转染组比,具有显著性差异(P<0.05)。[结论]Rac1shRNA载体pGPU6/GFP/Rac1-524通过使细胞伪足消失,可有效抑制卵巢癌细胞Skov3的迁移侵袭。
[Objective] To investigate the effect of Rac1 shRNA on the invasion ability of ovarian cancer cells Skov3. [Method] Rac1 shRNA vector pGPU6 / GFP / Rac1-524 was transfected into ovarian cancer cell line Skov3 by LipofectamineTM 2000, and the invasion and migration of transfected cells was detected by Transwell invasion assay. The ultrastructure of Skov3 cells was analyzed by scanning electron microscopy Structural changes. [Results] The migration distance of pGPU6 / GFP / Rac1-524 group was the shortest, the platelet pseudopods disappeared and the invasive ability decreased. Compared with the pGPU6 / GFP / Rac1-524 transfection group and the untransfected group, there was significant difference (P <0.05). [Conclusion] The Rac1 shRNA vector pGPU6 / GFP / Rac1-524 can effectively inhibit the migration and invasion of Skov3 in ovarian cancer cells by disappearing the pseudopodia.