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目的:建立怀药熟地黄中地黄苷A的反相高效液相色谱(RP-HPLC)含量测定法及薄层色谱(TLC)定性鉴别法。方法:采用RP-HPLC法对不同产地样品中地黄苷A成分的含量进行测定,采用TLC法进行定性鉴别。结果:HPLC含量测定地黄苷A在0.336~2.016μg范围内呈良好线性关系,r=0.999773,平均加样回收率为98.51%,RSD为1.23%(n=6),样品中地黄苷A精密度RSD为1.72%(n=6);采用该方法测定了10批次怀药熟地黄中地黄苷A。结论:所建方法快速、简便、准确,可作为怀药熟地黄中地黄苷A的定量控制方法,并建议怀药熟地黄按干燥品计算,含地黄苷A应不低于0.12%。
OBJECTIVE: To establish RP-HPLC method for the determination of midoside A of Rehmannia glutinosa Rehdirens and TLC qualitative identification method. Methods: The content of rehmannia glycoside A in different samples from different areas was determined by RP-HPLC method and qualitatively identified by TLC method. Results: The content of rehmannia glycosides A was linear in the range of 0.336 ~ 2.016μg with r = 0.999773, the average recovery was 98.51% and the RSD was 1.23% (n = 6) The RSD was 1.72% (n = 6). Ten batches of rehmannia glycosides of Rehmannia glutinosa were determined by this method. Conclusion: The method is rapid, simple and accurate. It can be used as a quantitative control method for the radix notogin A of Rehmannia glutinosa rhizome. And it is suggested that rehmannia glycyrrhizae A should be no less than 0.12% according to the dried products.