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目的 探讨卵巢上皮性癌病人nm2 3-H1基因蛋白表达与临床分期、分化程度及预后的关系及意义。方法 采用单克隆抗体免疫组织化学方法S -P法 ,对 70例卵巢上皮性癌手术标本经 10 %福尔马林固定 ,石蜡包埋组织进行nm2 3-H1基因蛋白表达的测定。结果 ⅠⅡ期卵巢上皮性癌nm2 3-H1基因蛋白表达阳性率为81 2 %。ⅢⅣ期阳性率为 46 3%,差异具有显著意义 (P <0 0 5 ) ,随着卵巢上皮性癌病理学分级的增高 ,nm2 3-H1基因蛋白表达阳性率下降 ,差异具有极其显著意义 (P <0 0 1)。ⅢⅣ期卵巢上皮性癌nm2 3-H1基因蛋白表达在生存期大于或等于 3年及小于 3年间差异具有极其显著的意义 (P <0 0 1)。结论 测定卵巢上皮性癌组织中nm2 3-H1基因蛋白表达 ,对判断预后有积极的作用。
Objective To investigate the relationship between nm23-H1 gene protein expression and clinical stage, differentiation and prognosis in patients with epithelial ovarian cancer. Methods The monoclonal antibody immunohistochemistry S-P method was used to determine the expression of nm23 3-H1 protein in 70 cases of epithelial ovarian cancer specimens fixed in 10% formalin and paraffin-embedded tissues. Results The positive rate of nm23-H1 gene in stage II ovarian epithelial carcinoma was 81.2%. The positive rate of stage ⅢⅣ was 46.3%, the difference was significant (P <0.05). The positive rate of nm23 3-H1 protein decreased with the increase of pathological grade of epithelial ovarian cancer, the difference was extremely significant P <0 0 1). The expression of nm23 3-H1 protein in stage ¢ ô ovarian epithelial carcinoma is extremely significant when the survival is greater than or equal to 3 years and less than 3 years (P <0.01). Conclusion The determination of nm23-H1 gene protein expression in epithelial ovarian cancer has a positive effect on prognosis.