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利用16SrDNA通用引物扩增了水稻褐飞虱Nilaparvata lugens(Stal)体内共生细菌的序列,经克隆、测序和NCBI数据库比对,发现褐飞虱体内存在杀雄菌属Arsenophonus类共生细菌,系统发育上与粉虱科和木虱科体内的Arsenophonus属亲源关系较近。在褐飞虱体内该共生细菌具有两种长度不同的16SrDNA序列,分别为1504bp和547bp,其中后者为前者中间缺失了957bp,其余序列相同。通过重新设计两对引物进行扩增,进一步确认不同褐飞虱地理种群及寄主种群均存在两种片段。Arsenophonus特异的23SrDNA引物的扩增结果表明,Arsenophonus存在于所有检测的褐飞虱种群中,但不存在于水稻寄主中。荧光定量PCR检测发现3个褐飞虱室内寄主种群Arsenophonus属共生细菌含量不同,其中TN1种群明显高于Mudgo种群和ASD7种群。此为水稻褐飞虱体内存在Arsenophonus属共生细菌的首次报道。
The 16S rDNA universal primers were used to amplify the sequence of the symbiotic bacteria in the brown planthopper Nilaparvata lugens (Stal). Cloning, sequencing and comparison with the NCBI database showed that there was a symbiotic bacterium of the genus Arsenophonus in the planthopper, And Psidodiaceae vivo Arsenophonus is close relatives. In the Nilaparvata lugens, the symbiotic bacteria have two 16S rDNA sequences with different lengths, which are 1504bp and 547bp respectively, of which the latter is 957bp deleted in the middle and the rest are the same. By redesigning two pairs of primers for amplification, it was further confirmed that there are two fragments of different BPH populations and host populations. Amplification of Arsenophonus-specific 23S rDNA primers indicated that Arsenophonus is present in all tested populations of BPH but not in rice hosts. Fluorescent quantitative PCR assay found that the content of Arsenophonus genus was different among the three hosts of Nilaparvata lugens, TN1 population was significantly higher than Mudgo population and ASD7 population. This is the first report of the presence of Arsenophonus symbiotic bacteria in rice brown planthopper.