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为满足生物医学领域对传感器灵敏度和特异性的要求,利用金纳米棒对纳米光纤消逝场强的消光作用,提出基于金纳米棒标记放大的锥形光纤生化传感器。通过理论计算金纳米棒对光纤传输特性的影响,设计实验证明纳米光纤具有对单个金纳米棒的响应能力。采用双抗夹心法对PBS溶液中的羊IgG检测,检测下限可达0.02ng/mL,证实此种传感器具有极高的灵敏度和特异性。提出的纳米光纤生化传感器结构简单、响应迅速、成本低廉,在医学临床诊断、食品安全检测、环境监测领域具有重要的实用价值。
In order to meet the sensitivity and specificity requirements of biomedical sensors, the extinction function of gold nanorods on the evanescent field of nanofibers was studied. A tapered optical fiber biosensor based on gold nanorods was proposed. The theoretical calculation of the effect of gold nanorods on the transmission characteristics of optical fibers shows that nanofibers have the ability to respond to single Au nanorods. Using double-antibody sandwich method for detection of goat IgG in PBS solution, detection limit of up to 0.02ng / mL, confirmed that this sensor has a high sensitivity and specificity. The proposed nanometer optical fiber biosensor has the advantages of simple structure, rapid response and low cost, and has important practical value in the fields of medical clinical diagnosis, food safety detection and environmental monitoring.