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目的 了解大黄乙醇提取物 (大黄 )体内抗单纯疱疹病毒 (HSV)的作用。方法 小鼠尾静脉接种滴度为 10 3TCID50 HSV Ⅰ型 0 15ml,第 2天皮下注射给药。将BALB c小鼠分为 7个组 ,分别给予不同剂量的药物 ,不同时间取肝、脾、肾等组织 ,做病理切片 ,观察病变情况 ;用空斑形成法滴定各脏器中的病毒滴度 ,观察药物在动物体内对HSV感染的抑制作用。结果 大黄乙醇提取物经皮下注射未发现小鼠有毒性反应 ;大黄各治疗组脾脏不出现病理变化 ,中、高剂量组肝脏、肾脏的病理损伤也能逐渐消失 ;大黄低剂量组与ACV阳性对照组效果相当 ;病毒滴度测定说明大黄能使各脏器中病毒滴度迅速下降 ,中、高剂量组比低剂量组作用明显 ;将各组数值进行两两比较 (Q检验 ) :F =4 9 14 5 9,P <0 0 0 1,总体均数差异有显著性 ;治疗组 (ACV、DH1、DH2、DH3)与非治疗组 (VC)差异有显著性 (P <0 0 1) ;DH2、DH3、与DH1差异有显著性 (P <0 0 1) ;DH1、DH2、DH3与ACV差异无显著性 (P >0 0 5 )。结果说明 ,对于总滴度平均值的下降 ,大黄与ACV同样有效 ,且中、高剂量组的效果优于低剂量组。结论 大黄乙醇提取物在体内具有非常明显的抗HSV感染作用 ,应用前景广阔。
Objective To understand the effect of rhubarb ethanol extract (rhubarb) against herpes simplex virus (HSV) in vivo. Methods The titer of tail vein of mice was 10 3TCID50 HSV type I 0 15 ml. The mice were given subcutaneous injection on the 2nd day. The BALB c mice were divided into 7 groups and given different doses of drugs. The liver, spleen, kidney and other tissues were taken at different times to make pathological sections and the lesions were observed. The virus titration in each organ was punctured by plaque formation method. Degree, to observe the inhibitory effect of the drug on HSV infection in animals. RESULTS: No toxic reaction was found in the mice by subcutaneous injection of rhubarb ethanol extract; no pathological changes occurred in the spleen of the rhubarb treatment groups, and the pathological lesions of the liver and kidneys were gradually disappeared in the middle and high dose groups; the low dose group of rhubarb and ACV positive controls were gradually disappeared. The group effect was comparable; Virus titer assay showed that the rhubarb can rapidly reduce the virus titer in all organs, and the effect of the middle and high dose groups was significantly higher than that of the low dose group; the values of each group were compared (Q test): F = 4 9 14 5 9, P <0 0 1 1. There was a significant difference in the mean mean; there was a significant difference between the treatment group (ACV, DH1, DH2, DH3) and non-treatment group (VC) (P <0 01). There was significant difference between DH2, DH3, and DH1 (P < 0 01); there was no significant difference between DH1, DH2, DH3, and ACV (P > 0 05). The results showed that rhubarb was as effective as ACV in reducing the mean value of total titer, and the effect of middle and high dose group was better than that of low dose group. Conclusion Ethanol extract of rhubarb has very obvious anti-HSV infection in vivo and has broad application prospects.