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为了避免现行封闭抗体检测方法的不合理性,对其进行改进,将男方刺激细胞与妇女待测血清孵育,然后分离结合了封闭抗体的刺激细胞,将经或未经封闭抗体作用的男方刺激细胞分别与女方反应细胞在25%正常AB型血清同等培养条件下培养6d.改进后方法测得正常生育组与原发性反复自然流产组封闭效率阳性率为57.1%及12.3%,均数为7.53±10.30及—55.51±13.41.改进后方法的准确度(81.7%)、灵敏度(87.8%)及特异性(57.1%)均高于改进前.在分析白细胞免疫疗法免疫效果时发现,改进后的方法更能准确反应患者体内封闭效率逐渐上升趋势.改进后的封闭效率在评价反复自然流产治疗的临床疗效时优于改进前.
In order to avoid the irrationality of the current detection methods of blocking antibodies, it is improved, and the male-stimulating cells are incubated with the serum to be tested by the women. Then, the stimulating cells that bind to the blocking antibodies are isolated and stimulated by the man with or without the blocking antibody Respectively with female reactive cells cultured in 25% normal AB type serum under the same conditions for 6 days.The improved method was measured in normal and primary recurrent spontaneous abortion group, the positive rate of blocking efficiency was 57.1% and 12.3%, the average was 7.53 ± 10.30 and -55.51 ± 13.41 respectively.The accuracy of the improved method (81.7%), sensitivity (87.8%) and specificity (57.1%) were higher than those before the improvement.When analyzing the immune effect of leukocyte immunotherapy, we found that the improved The method can accurately reflect the gradual increase of the blocking efficiency in patients.The improved efficiency of closure is better than that of the improved one in evaluating the curative effect of recurrent spontaneous abortion.